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Forum: Sample Prep / Library Generation 10-01-2019, 11:02 AM
Replies: 1
Views: 2,523
Posted By SCIL2019
The transposon showed by Epicentre is mainly for...

The transposon showed by Epicentre is mainly for mutagenesis. For NGS applications you load Ez-Tn5 as done for any other (Picelli et al, 2014 for example) using the P5/MErev and P7/MErev. You can do...
Forum: Illumina/Solexa 10-01-2019, 10:56 AM
Replies: 11
Views: 1,965
Posted By SCIL2019
Ez-Tn5

Does anybody know the loading protocol for this enzyme?
Forum: Sample Prep / Library Generation 09-14-2019, 03:35 PM
Replies: 20
Views: 7,224
Posted By SCIL2019
Thanks J for your comment.! If you look...

Thanks J for your comment.!

If you look carefully the size distribution you refer is there, just not well defined. 10x has indicated this is something they have observed also. This seems to happen...
Forum: Sample Prep / Library Generation 09-13-2019, 02:32 PM
Replies: 20
Views: 7,224
Posted By SCIL2019
Larger fragemtns atac

Hi all,

I am having a bit of an issue with this library/sample. I am getting larger fragments peak and then 200 bp peak is gone. This is using 10x ATAC kit.

I usually don;t see such a nhigfh...
Forum: Sample Prep / Library Generation 05-07-2019, 12:32 PM
Replies: 0
Views: 770
Posted By SCIL2019
Lightbulb ATAC Library from ffpe

Hi all,

Does anyone has an example of bioanalyzer traces of ATAC libraries prepared from ffpe?

Thank you!

SCIL2019
Forum: Introductions 05-07-2019, 12:26 PM
Replies: 3
Views: 1,663
Posted By SCIL2019
Thank you! I’ll do!

Thank you! I’ll do!
Forum: Introductions 05-07-2019, 01:23 AM
Replies: 3
Views: 1,663
Posted By SCIL2019
Lightbulb Just wanted to say Hi and ask a question

Dear Fellow Seqs, I am new here. Itís a pleasure to know you have my back. I hope we can have fun.

After my short intro, I was wondering if you happen to have an example of a bioanalyzer trace...
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