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Forum: Bioinformatics 01-10-2018, 11:42 PM
Replies: 5
Views: 986
Posted By raman91
Thanks for your reply. I am pretty sure that the...

Thanks for your reply. I am pretty sure that the reads are mapped to hg19 build only. I checked the IGV browser too and the read sequence match to the reference sequence.
Forum: Bioinformatics 01-09-2018, 01:20 AM
Replies: 5
Views: 986
Posted By raman91
Iím sorry, I didnít explain the issue properly....

Iím sorry, I didnít explain the issue properly. Iím working on CG WGS data with avg read depth of 40x. The QC metrics looks good for all parameters and the alignment rate is 97.43%. However, I had...
Forum: Bioinformatics 01-02-2018, 11:44 PM
Replies: 5
Views: 986
Posted By raman91
No coverage or very low coverage in the Complete Genomics data

Hi,

Has anyone worked on Complete Genomics data? Most of the exons in the data have no coverage or very low coverage (5-10 reads only) even though the reads are sequenced at 40X coverage. Can...
Forum: General 12-27-2017, 02:50 AM
Replies: 0
Views: 1,124
Posted By raman91
Insert Size in IGV ?

Hi,

I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and...
Forum: De novo discovery 12-27-2017, 02:33 AM
Replies: 3
Views: 7,030
Posted By raman91
Hi, I am visualizing alignments using IGV....

Hi,

I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and...
Forum: Bioinformatics 12-27-2017, 02:28 AM
Replies: 0
Views: 666
Posted By raman91
IGV Insert size

Hi,

I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and...
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