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Forum: Illumina/Solexa 07-05-2016, 11:50 AM
Replies: 1
Views: 1,503
Posted By rgejman
Distance between P5 and custom read 1 primer

Dear all,

I have a minigene library which is amplified with P5/P7 sites that looks like this

[P7/barcode][constant region][ variable region ][18nt constant region][SP1][P5] (436nt total)

We...
Forum: Illumina/Solexa 07-13-2011, 11:07 AM
Replies: 5
Views: 6,001
Posted By rgejman
Does this "double priming" have to happen once or...

Does this "double priming" have to happen once or every time you load a v1 vs. v3 flow cell? I actually buy 200 cycle kit (actually 202 cycle kits) and aliquot them into 4x 50 cycle kits (for which I...
Forum: Illumina/Solexa 07-12-2011, 04:08 PM
Replies: 5
Views: 6,001
Posted By rgejman
TruSeq v3 Cluster Kits with TruSeq SBS kits?

Hi all,

Has anyone experimented with using TruSeq SBS kits (i.e. not v3 SBS kits) to sequence TruSeq v3 Cluster Kits and flow cells?. I have heard through the grapevine that it can be done and I...
Forum: Bioinformatics 11-15-2010, 07:47 AM
Replies: 11
Views: 7,115
Posted By rgejman
Has anyone resolved this issue? I am still seeing...

Has anyone resolved this issue? I am still seeing disconnected transcripts in many genes using tophat v1.1.2 and cufflinks 0.9.2. Tophat is run without the -G option, so de novo transcripts are found...
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