Forum: Bioinformatics
06-28-2016, 10:06 AM
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Replies: 5
Views: 1,856
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Forum: Bioinformatics
06-20-2016, 07:55 AM
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Replies: 5
Views: 1,856
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Forum: Bioinformatics
06-16-2016, 09:57 AM
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Replies: 5
Views: 1,856
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Forum: Bioinformatics
06-16-2016, 09:34 AM
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Replies: 5
Views: 1,856
dbSNP database for just NA12878
So I may be completely wrong here but please correct me since I'm mostly used to doing RNA splicing analysis.
I have sequenced sheared DNA from about 10 NA12878 cells using Illumina and a library...
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Forum: Bioinformatics
07-30-2015, 07:01 AM
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Replies: 12
Views: 7,371
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Forum: Bioinformatics
07-30-2015, 06:46 AM
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Replies: 5
Views: 4,332
Hi nucacidhunter,
Thank you for your input....
Hi nucacidhunter,
Thank you for your input. What I've seen many people do out there is read the UMI as part of their DNA library insert and not as part of the index. I still don't know how Agilent...
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Forum: Bioinformatics
07-28-2015, 09:02 AM
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Replies: 5
Views: 4,332
Hi sudders,
From my very limited experience...
Hi sudders,
From my very limited experience with random barcodes, I've heard that this bias could be due to 2 reasons.
a) Synthesis bias. Some oligo companies suggest manually mixing the 4 bases...
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