Forum: Pacific Biosciences
02-25-2013, 07:55 AM
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Replies: 14
Views: 7,500
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Forum: Pacific Biosciences
02-21-2013, 06:53 PM
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Replies: 14
Views: 7,500
PacBio read lengths.. How long?
Lately we've been running a number of 16-20kb libraries using XL enzyme and C2 chemistry. We're getting 30-35kb reads, and 25kb subreads... Only a few per run, but they definitely improve assembly....
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Forum: Pacific Biosciences
02-21-2013, 06:41 PM
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Replies: 12
Views: 5,358
The # of productive ZMWs is influenced by the...
The # of productive ZMWs is influenced by the size of the library. We regularly get 50-55% with 2-6kb libraries, for 16kb libraries we can't get above 33%.... Lower than I'd like, but the reads are...
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Forum: Illumina/Solexa
07-13-2011, 07:21 AM
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Replies: 5
Views: 6,001
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Forum: Sample Prep / Library Generation
08-09-2010, 07:56 AM
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Replies: 0
Views: 2,601
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Forum: Sample Prep / Library Generation
08-09-2010, 07:50 AM
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Replies: 13
Views: 7,851
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Forum: De novo discovery
11-16-2009, 12:40 PM
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Replies: 1
Views: 4,743
de novo assembly and Illumina read length
We are preparing to do paired-end de novo sequencing on our GAIIx with the new kits. Is there a reason to run less than the maximum 100+ cycles on each end? There was a suggestion that the higher...
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Forum: Illumina/Solexa
04-29-2009, 06:27 PM
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Replies: 4
Views: 2,386
I don't think I have ever ordered items w/...
I don't think I have ever ordered items w/ delivery in less than 2 weeks, then delayed further. I have found that by kindly 'badgering' my sales rep and FAS I can get partial delivery to keep me going
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Forum: Illumina/Solexa
05-23-2008, 05:00 AM
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Replies: 4
Views: 3,791
Interesting.....I looked at the website that...
Interesting.....I looked at the website that shows the primer sequences, they appear to be different for all the kits - but I have used the same primer for PhiX, smRNA, and genomic sequencing. I...
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Forum: Illumina/Solexa
05-22-2008, 03:51 PM
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Replies: 4
Views: 3,791
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Forum: General
05-22-2008, 03:48 PM
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Replies: 3
Views: 3,938
Before jumping in you should definitely get a...
Before jumping in you should definitely get a sense of what the PIs would get the most out of. While they are both considered next gen systems, short read and long read sequences are often very...
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