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Forum: RNA Sequencing 07-12-2019, 03:24 PM
Replies: 0
Views: 603
Posted By bzguy
Unhappy tximport problem - won't recognize when there is an isoform and throughout data

Hi,
I was hoping someone can please help me with an issue I can’t really figure out…

I ran samples on Salmon through Galaxy. I also provided a conversion file, with transcript name and gene ID...
Forum: Bioinformatics 10-01-2018, 09:14 AM
Replies: 0
Views: 475
Posted By bzguy
Strand information for alignment of KAPA mRNA HyperPrep Kit data

Hello,
I am looking on data generated with KAPA mRNA HyperPrep Kit. this library prep is stranded. When I run Salmon/Histat2 for alignment, it asks whether the data is...
Forum: RNA Sequencing 09-19-2018, 05:02 PM
Replies: 1
Views: 1,706
Posted By bzguy
featureCounts - does it normalize by gene length?

Hello,
I'm starting to analyze RNA seq data, and I was hoping you can please help me clear something up

I am using FASTQ groomer, then TopHat2 to align, and then featureCounts, to get a number of...
Forum: Illumina/Solexa 06-17-2011, 06:02 AM
Replies: 0
Views: 803
Posted By bzguy
The Imaging Compartment

Hello everyone,
Does anyone have documentations/slides which talk about how the Imaging compartment of the Genome Analyzer works?

I am looking for some information and sketches of where the...
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