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Forum: Sample Prep / Library Generation 12-13-2019, 03:25 AM
Replies: 1
Views: 668
Posted By Shahrukh
So this week, I tried sonication with Sonics...

So this week, I tried sonication with Sonics Vibra cell 500/750. I want to share my experience and hopefully get input from you. The details are as follows:
- ~3m HEK293T cells per sample
- 500ul...
Forum: Sample Prep / Library Generation 12-09-2019, 06:35 AM
Replies: 1
Views: 668
Posted By Shahrukh
Post Shearing chromatin - ChIP-seq for H3K9me3

Hi everyone,
It's my first time posting on seqanswers.
I am shearing chromatin of HEK293T ~5m cells. I am using the ChIP-seq protocol from Myers lab/Encode consortia. Looking at 200-600bp sheared...
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