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Search: Posts Made By: vehuardo
Forum: Illumina/Solexa 09-18-2015, 01:01 AM
Replies: 3
Views: 1,769
Posted By vehuardo
Thanks Bryanbriney. These were whole-genome...

Thanks Bryanbriney.

These were whole-genome frgmented DNA libraries prepared using Kapa + bioo scientific indexes. You are probabbly spot on. There were some adapter-dimers and adapters visible on...
Forum: Illumina/Solexa 09-18-2015, 12:43 AM
Replies: 3
Views: 1,769
Posted By vehuardo
MiSeq: Sudden drop in %> Q30 halfway read 1

We have a run going on the MiSeq as we speak (2x250 bp). I just had a look at the run in the SAV.

A sudden drop in Q30 after about 75 cycles caught my attention. Has anyone seen anything like...
Forum: Ion Torrent 02-04-2015, 02:47 AM
Replies: 121
Views: 100,136
Posted By vehuardo
Thanks Tracer, This answers all my...

Thanks Tracer,

This answers all my questions. Brilliant!

Regarding this:

"Bottom line:
If you have a large volume of samples that arrive continuously (so you can maximize the throughput of...
Forum: Ion Torrent 02-02-2015, 04:20 AM
Replies: 121
Views: 100,136
Posted By vehuardo
Thanks for the info!

Thanks for the info!
Forum: Ion Torrent 01-27-2015, 03:31 AM
Replies: 121
Views: 100,136
Posted By vehuardo
Hi all! My institute will purchase a...

Hi all!


My institute will purchase a benchtop sequencer for bacterial genome sequencing. It will be used both for routine genome seq and (importantly) for infetious disease outbreak analyses....
Forum: Sample Prep / Library Generation 03-21-2014, 10:43 AM
Replies: 8
Views: 4,776
Posted By vehuardo
I have used NEBnext fragmentase for approx 300...

I have used NEBnext fragmentase for approx 300 bacterial genomes. Works well!
Forum: RNA Sequencing 10-10-2013, 11:51 AM
Replies: 9
Views: 8,806
Posted By vehuardo
Thanks for your quick response, James. Thats very...

Thanks for your quick response, James. Thats very informative. Surprisingly hard to come by any clear advice or common views on this topic.

Looking forward to the paper!

Cheers
Forum: RNA Sequencing 10-07-2013, 06:58 AM
Replies: 9
Views: 8,806
Posted By vehuardo
50 bp single end sequencing

Hi Torst,

I see this reply of yours is rather old, but I´d like to follow up on this: do you use 50 bp SE sequencing for bacterial RNA-seq?

I am doing some bacterial RNA-seq myself, ran a few...
Forum: Illumina/Solexa 05-23-2013, 01:35 AM
Replies: 21
Views: 13,623
Posted By vehuardo
BTW, gel image Couldn´t upload: too big....

BTW, gel image

Couldn´t upload: too big. I´ll try again later, have no image edit software on this computer...
Forum: Illumina/Solexa 05-23-2013, 01:30 AM
Replies: 21
Views: 13,623
Posted By vehuardo
Thanks for your reply! I actually prepped 8...

Thanks for your reply!

I actually prepped 8 M. tuberculosis illumina libraries yesterday, as a preliminary test. Fragmentation was carried out with fragmentase. After final amplification and...
Forum: Illumina/Solexa 05-22-2013, 12:04 PM
Replies: 21
Views: 13,623
Posted By vehuardo
Still no one get this fragmentase to work?

Hi all,

seems like the NEBNext fragmentase isn´t the most popular enzyme in town.. Is there anyone in here who actually successfully use fragmentase on a routine basis?
I had planned to use it...
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