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Search: Posts Made By: rbstruijk
Forum: SOLiD 05-16-2012, 12:52 AM
Replies: 4
Views: 3,688
Posted By rbstruijk
Thanks JPC, it looks like only the adapters are...

Thanks JPC, it looks like only the adapters are in fact nick translated.
Forum: SOLiD 05-15-2012, 07:09 AM
Replies: 4
Views: 3,688
Posted By rbstruijk
Above question is about the Solid 5500...

Above question is about the Solid 5500 single-ended library prep
Forum: SOLiD 05-15-2012, 07:08 AM
Replies: 4
Views: 3,688
Posted By rbstruijk
Library prep question - ligation of P1 and P2 adapters

Hi there,

I'm trying to grasp how the nick translation works exactly after ligating the P1 and P2 adapters to A-tailed DNA fragments. Does the reaction at 72oC exchange the nucleotides of the...
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