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Search: Posts Made By: Emanuel Heitlinger
Forum: Bioinformatics 12-10-2011, 11:02 PM
Replies: 3
Views: 3,923
Posted By Emanuel Heitlinger
Did I misunderstand "if both of the factors in my...

Did I misunderstand "if both of the factors in my model [...] explain a significant proportion of the variance in the model" ?

I thought Seanna wanted to know how much variance the significant...
Forum: Bioinformatics 12-09-2011, 05:59 AM
Replies: 3
Views: 3,923
Posted By Emanuel Heitlinger
Hi Seanna, Not sure wether this is really...

Hi Seanna,
Not sure wether this is really answering you questions, but I have fun at the moment playing around with the R-package vegan:
You can use principal component analysis (e.g. in the rda...
Forum: 454 Pyrosequencing 01-21-2010, 11:54 PM
Replies: 29
Views: 12,670
Posted By Emanuel Heitlinger
donniemarco, did you read the whole thread?...

donniemarco,
did you read the whole thread? Look at my post about progblems with cap within tgicl! If yours are is the same problem, use the solution Sklages provided, it worked for me and for at...
Forum: 454 Pyrosequencing 11-20-2009, 07:49 AM
Replies: 34
Views: 14,494
Posted By Emanuel Heitlinger
Does Roche make the new version of newbler...

Does Roche make the new version of newbler available to users of 454 data?
Forum: 454 Pyrosequencing 05-24-2009, 05:09 AM
Replies: 34
Views: 14,494
Posted By Emanuel Heitlinger
Wowwww I just found all this: ...

Wowwww
I just found all this:
http://www.bio.utexas.edu/research/matz_lab/matzlab/Methods.html
Forum: 454 Pyrosequencing 05-21-2009, 01:04 PM
Replies: 26
Views: 41,600
Posted By Emanuel Heitlinger
hey Stella, you got mail! see you in...

hey Stella,

you got mail!
see you in Edinburgh!

Is Nicola already on her maternity leave?
Forum: Introductions 05-21-2009, 04:21 AM
Replies: 373
Views: 120,273
Posted By Emanuel Heitlinger
Hi, I am a PhD student sequencing the...

Hi,

I am a PhD student sequencing the transcriptome of a parasitic nematode using 454. Later on I will be doing digital transcriptomics (gene expression) using solexa.


Cheers,
Emanuel
Forum: 454 Pyrosequencing 05-21-2009, 03:20 AM
Replies: 29
Views: 12,670
Posted By Emanuel Heitlinger
Thanks! I just downloaded the right binay,...

Thanks!

I just downloaded the right binay, copied it to tgicl/bin and voila: It works! The clustering takes only very short time running on 6 processors... I still have to wait wait till CAP3...
Forum: 454 Pyrosequencing 05-20-2009, 07:05 AM
Replies: 29
Views: 12,670
Posted By Emanuel Heitlinger
Hi, I am just following kmcarr's advice...

Hi,

I am just following kmcarr's advice using TGICL to cluster ~75.000 ~250nt sequences from a GS FLX run.

CAP3 has a problem, giving the following error:

*** glibc detected *** cap3:...
Forum: 454 Pyrosequencing 05-20-2009, 04:17 AM
Replies: 34
Views: 14,494
Posted By Emanuel Heitlinger
Hi all, the Evrogen MINT kit worked better than...

Hi all,
the Evrogen MINT kit worked better than the SMART kit for me. The SMART kit produced strange concatenated primer/adaptor artefacts. Currently it is used without any polyT modifications...
Forum: 454 Pyrosequencing 03-16-2009, 05:21 AM
Replies: 12
Views: 6,625
Posted By Emanuel Heitlinger
Syntax error: "(" unexpected

Same Problem as coldturkey
/opt/454/apps/assembly/bin/gsAssembler: 114: Syntax error: "(" unexpected

Most likely reason:
On Ubuntu the .rpm files are not installed! The GUI needs some of them. I...
Showing results 1 to 11 of 11

 


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