SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 5 of 5
Search took 0.00 seconds.
Search: Posts Made By: skm
Forum: Bioinformatics 07-03-2017, 09:48 AM
Replies: 1
Views: 567
Posted By skm
Smile [ddRAD] on removal of duplicate reads

Hi All,

Is it advisable to remove duplicate reads from double digest RAD (ddRAD) data generated on Illumina Hiseq platform ?

Regards,
Krishna
Forum: Bioinformatics 10-16-2013, 09:16 PM
Replies: 0
Views: 1,833
Posted By skm
cuffmerge assembly vs denovo assembly of RNAseq data

Hi all,

I have RNAseq data of stress-control of various tissue of a plant. I am doing differential gene expression. In addition, cuffmerge provides me with transcripts assembled. But is this a ...
Forum: Bioinformatics 08-23-2013, 06:50 AM
Replies: 0
Views: 1,296
Posted By skm
Smile tophat/cufflink slog fold change +/- inf

Dear all,

I am using latest tophat/cufflinks suite.

A differentially expressed gene has FPKM values of 0 and 34 in two samples A and B. The log2fold change gives me +infinity. Similarly, 28...
Forum: RNA Sequencing 04-30-2013, 02:14 AM
Replies: 4
Views: 2,034
Posted By skm
The organism is a plant (Cajanus cajan). Any...

The organism is a plant (Cajanus cajan).
Any clues ?

Thanks!
SKM
Forum: RNA Sequencing 04-30-2013, 12:36 AM
Replies: 4
Views: 2,034
Posted By skm
RNA-seq: how many million reads are required ?

Hi all,

I am having difficulty in assessing how many million reads are required to optimally capture a transcriptome ?.

I have typically 13 million reads from a Miseq run for 7 samples (1.85...
Showing results 1 to 5 of 5

 


All times are GMT -8. The time now is 06:35 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO