Forum: Bioinformatics
01-13-2011, 08:27 AM
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Replies: 2
Views: 3,340
Why is ML/NJ phylogeny different from SNP tree?
Greetings.
I am working on a bacterial genome. We have determined the complete genome sequence of this genome and want to study the evolutionary relationship of my genome and its VERY CLOSE...
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Forum: Bioinformatics
09-14-2010, 09:32 PM
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Replies: 5
Views: 2,712
Dear all,
Many thanks for your wonderful...
Dear all,
Many thanks for your wonderful replies. ALL are helpful! I have calculated dN/dS either and all results are reasonable, but could be slightly different between genomes.
This is my...
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Forum: Bioinformatics
09-12-2010, 08:24 PM
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Replies: 5
Views: 2,712
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Forum: 454 Pyrosequencing
04-09-2009, 08:36 AM
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Replies: 2
Views: 2,961
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Forum: 454 Pyrosequencing
04-08-2009, 11:59 AM
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Replies: 2
Views: 2,961
454 titanium sequence company
Hello, we want to sequence whole genome on 454 titanium platform in a sequence company. Can any body recommend a company besides 454 life science to us?
Thanks a lot.
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Forum: 454 Pyrosequencing
03-19-2009, 08:33 AM
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Replies: 2
Views: 1,873
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Forum: 454 Pyrosequencing
03-18-2009, 02:36 PM
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Replies: 2
Views: 1,873
Want to generate more data to fill in gaps
Hello,
This might be a stupid question.
We are currently working on a ~5M bacterial genome assembly. Our reads data is ~8X coverage, 454 sequencing. The coverage is kind of low. So we used...
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Forum: 454 Pyrosequencing
02-04-2009, 09:34 AM
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Replies: 4
Views: 4,630
454 sequencing data coverage
Dear All,
I am currently working on 454 sequencing data. The average length of reads is ~200bp, and coverage is ~8-10X.
Does anybody tell me how the data coverage is? Is it ok or not?...
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Forum: 454 Pyrosequencing
01-22-2009, 09:13 AM
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Replies: 3
Views: 4,951
Homopolymer error in 454 sequencing data
Hello,
I am currently working on genome assembly. our reads data is from 454 sequencing. First we assembled contigs using Newbler. However, when we compared our built genome with a reference genome...
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