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Forum: Sample Prep / Library Generation 08-21-2019, 08:17 AM
Replies: 0
Views: 352
Posted By MollySchools
Phusion Reaction

My lab is working on a simple library prep and are running into an issue with our amplification PCR, our samples do not successfully amplify. After preforming the reaction (using 2X Phusion HF...
Forum: Sample Prep / Library Generation 08-19-2019, 06:21 AM
Replies: 2
Views: 610
Posted By MollySchools
Hi Luc, Thank you for your reply. I have also...

Hi Luc,
Thank you for your reply. I have also tried to use Kapa Hifi and have seen the same results. I am using a qubit to measure concentrations. While 12 cycles may be to much for the samples...
Forum: Sample Prep / Library Generation 08-15-2019, 01:49 PM
Replies: 2
Views: 610
Posted By MollySchools
Pre-Hybridization PCR not running efficiently

Hello all,
I am working on building my libraries for an NGS project. I am running into a problem in which my pre-amplified libraries have concentrations ranging from 8 ng/uL up to 15 ng/uL....
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