SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 8 of 8
Search took 0.00 seconds.
Search: Posts Made By: kshazand
Forum: Illumina/Solexa 01-08-2019, 12:15 PM
Replies: 2
Views: 804
Posted By kshazand
Thanks for the reply. If there is still a bias,...

Thanks for the reply. If there is still a bias, there should be a heterogeneous coverage. Are you comfortable with that?
Forum: Illumina/Solexa 12-13-2018, 10:46 AM
Replies: 2
Views: 804
Posted By kshazand
Nextera DNA Flex performance

Hi,
I wonder if someone can share some library QC information (Picard type) on the new Nextera DNA Flex, such as dupe rate, estimated library size, GC bias, etc. This needs to be without enrichment,...
Forum: Illumina/Solexa 06-05-2015, 10:21 AM
Replies: 3
Views: 1,297
Posted By kshazand
Thanks Brian. We have moved to V2 but the...

Thanks Brian. We have moved to V2 but the analyzed data were from the V1 period.
Is there an email where I can send you a slide?
Forum: Illumina/Solexa 06-05-2015, 05:55 AM
Replies: 3
Views: 1,297
Posted By kshazand
NextSeq data bias

Hi,
We notice an unexpected variation of the estimated library size as a function of the number of reads, even after downsampling with NextSeq data. This is not observed with MiSeq-generated...
Forum: Sample Prep / Library Generation 03-25-2014, 06:00 AM
Replies: 14
Views: 8,674
Posted By kshazand
What enzymes are they then?

What enzymes are they then?
Forum: Sample Prep / Library Generation 03-25-2014, 06:00 AM
Replies: 14
Views: 8,674
Posted By kshazand
luc, Ppl looked into sonicated DNA and it does...

luc,
Ppl looked into sonicated DNA and it does have all sorts of damages, hence my question.
Forum: Sample Prep / Library Generation 03-20-2014, 12:14 PM
Replies: 14
Views: 8,674
Posted By kshazand
Yes thank you, I know Fragmentase, but since it...

Yes thank you, I know Fragmentase, but since it is mainly based on restriction enzymes, the output has some bias. What other "many alternatives" are you refering to?
Forum: Sample Prep / Library Generation 03-20-2014, 07:32 AM
Replies: 14
Views: 8,674
Posted By kshazand
DNA fragmentation methods

Hi all,
Different physical damages are made to DNA during sonication. Yet, we all use Covaris and other similar sonicators for library prep.
I would like to know how many of my colleagues have...
Showing results 1 to 8 of 8

 


All times are GMT -8. The time now is 08:05 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO