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Forum: Sample Prep / Library Generation 08-24-2020, 04:57 AM
Replies: 1
Views: 615
Posted By ETBA
i5 / i7 dual indexing + ATAC

Hello,

I am constructing an ATAC-seq library using the standard Illumina kit (Nextera DNA Flex Library Prep, FC-121-1030). A previous user suggested dual indexing to minimise index hopping which I...
Forum: Sample Prep / Library Generation 08-03-2020, 03:47 PM
Replies: 4
Views: 900
Posted By ETBA
Brilliant that was very helpful. Although a bit...

Brilliant that was very helpful. Although a bit of shame as many samples are already done on a single indexing strategy with i7 so i'll have to stick with that. I'll certainly consider the use of a...
Forum: Sample Prep / Library Generation 08-03-2020, 09:21 AM
Replies: 4
Views: 900
Posted By ETBA
That's great, thanks for the information. Just to...

That's great, thanks for the information. Just to be safe, i'm assuming that the dual indexes are also the oligos specified in Buenrostro, 2013?
Forum: Sample Prep / Library Generation 08-03-2020, 08:15 AM
Replies: 4
Views: 900
Posted By ETBA
Sequencing Human/Mouse ATAC libraries together?

Hi all,

I am a novice to this and currently preparing mouse and human ATAC libraries. All samples are on different barcodes and we want to compare these samples, so we think it's better to...
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