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Search: Posts Made By: iramai
Forum: Epigenetics 07-13-2020, 06:28 AM
Replies: 37
Views: 36,495
Posted By iramai
Hi Felix, Thanks for answering so fast. I...

Hi Felix,
Thanks for answering so fast.
I have been testing some of your tips from the link you shared on the previous comment, but I can't obtain higher alingment rates.

First I change the...
Forum: Epigenetics 07-06-2020, 08:22 AM
Replies: 37
Views: 36,495
Posted By iramai
Hi all, Maybe I am too late for posting here...

Hi all,
Maybe I am too late for posting here the same problem as all you did 6 years before.

I am trying to analyze some bisulfite sequencing samples with bismark/bowtie2, and I also get really...
Forum: Bioinformatics 05-26-2020, 05:43 AM
Replies: 0
Views: 698
Posted By iramai
GRCm38 and mm10 reference genomes, Which one to use?

Hy everybody!
I have received the fastq files of some Bisulfite sequencing samples. For the sequencing they have used "gem3.mmusculus.GRCm38_BS" as a reference. But I am not sure what genome to use...
Forum: Bioinformatics 05-17-2020, 07:04 AM
Replies: 3
Views: 833
Posted By iramai
Thanks for your answer! As the only information...

Thanks for your answer!
As the only information of the sequencing service was that about the dual index adapters I though that those were the sequences that I need to trim.
So for the command how...
Forum: Bioinformatics 05-16-2020, 12:50 PM
Replies: 3
Views: 833
Posted By iramai
How to trim dual index adapters with Trim Galore!

Hi everybody,
I am trying to trim some adapters with Trim Galore from my bisulfite sequencing data, but I don't know what command to use.
It has been really difficult to take the information of...
Forum: Epigenetics 03-16-2018, 06:58 AM
Replies: 0
Views: 4,307
Posted By iramai
Extract the coordinates of the gaps between peaks.

Hi everybody, i'm new in SeQanswers.
I have some sequencing data from GEO repository it is written like this:

chr1 1 2001 int
chr1 1001 3001 int
chr1 2001 4001...
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