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Forum: Sample Prep / Library Generation 10-14-2015, 04:47 AM
Replies: 2
Views: 1,758
Posted By Martin Mikkelsen
We have seen the same, but never really got an...

We have seen the same, but never really got an answer from Illumina
Forum: Sample Prep / Library Generation 11-25-2013, 11:56 PM
Replies: 1
Views: 2,209
Posted By Martin Mikkelsen
I solved the problem (well somebody in the lab...

I solved the problem (well somebody in the lab helped) me.

We used heating in thermocycler lid. Somehow it affected the incubations steps. Now we dropped the temperature in the lid and everything...
Forum: Sample Prep / Library Generation 11-18-2013, 06:37 AM
Replies: 1
Views: 2,209
Posted By Martin Mikkelsen
Problems getting results using Illumina / NEB kit for small RNA library prep.

Dear all,

Does anyone have any experience with these kits?

I was able to make one successful build using the Illumina kit and since then has everything failed. I have also tried the similar kit...
Forum: Illumina/Solexa 11-11-2013, 06:12 AM
Replies: 16
Views: 12,757
Posted By Martin Mikkelsen
Does anyone have experience using a LabChip XT...

Does anyone have experience using a LabChip XT for this?

We are thinking of using one, but we need to figure out the correct setting. We have tried the application note from PerkinElmer but we...
Forum: 454 Pyrosequencing 02-26-2013, 12:57 AM
Replies: 1
Views: 1,921
Posted By Martin Mikkelsen
Help on library, restriction enzyme fragmentation

Dear Everyone,

At our lab we are doing a sequencing project sequencing a short gene (1500 kb) in humans. The length of the amplicon does not really fit any of the fragmentations tools available in...
Forum: 454 Pyrosequencing 04-24-2012, 11:54 PM
Replies: 4
Views: 2,339
Posted By Martin Mikkelsen
We got it a week ago from our product specialist....

We got it a week ago from our product specialist. I was told that there would be a new version ultimo March
Forum: 454 Pyrosequencing 04-24-2012, 12:45 AM
Replies: 4
Views: 2,339
Posted By Martin Mikkelsen
The software package GS Data Analysis 2.7 on Ubuntu

Have anyone tried the 2.7 version of the software on Ubuntu?

I have it installed everything and I am able to open the GUI. But I cannot add a SFF file to a project.

I can open old runs and...
Forum: 454 Pyrosequencing 10-19-2010, 12:52 AM
Replies: 7
Views: 2,609
Posted By Martin Mikkelsen
So as for anything in life, the bigger the better...

So as for anything in life, the bigger the better :-)
Forum: 454 Pyrosequencing 10-18-2010, 10:43 AM
Replies: 7
Views: 2,609
Posted By Martin Mikkelsen
Hi Zaag, Thanks for your reply. The...

Hi Zaag,

Thanks for your reply.

The plan is mostly shotgun de novo sequencing, so I guess quadcore is not that important?
Forum: Introductions 10-18-2010, 05:09 AM
Replies: 0
Views: 1,051
Posted By Martin Mikkelsen
Smile Hello from Denmark!

Hi everyone,

Just a quick introduction.

We are a genetic lab in Denmark that just bought a GS Junior (Roche).

Currently the plan is do research on it. We are starting out with full human...
Forum: 454 Pyrosequencing 10-18-2010, 05:04 AM
Replies: 7
Views: 2,609
Posted By Martin Mikkelsen
Computer for running GS Junior Software

Dear all,

We just bought a GS Junior at my lab. We are planning to do most of the data analysis outside the lab and away from the computer that comes with the instrument.

Since my computer is...
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