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Forum: Sample Prep / Library Generation 09-11-2012, 01:41 AM
Replies: 3
Views: 3,315
Posted By vvendr
I've tried both protocols and for me the best one...

I've tried both protocols and for me the best one is the NuGEN Ovation kit v2. You need only 500pg RNA to start and in few hours you are able to amplify it. I think it is also more user-friendly...
Forum: Illumina/Solexa 02-10-2012, 10:01 AM
Replies: 22
Views: 12,310
Posted By vvendr
Finally, I asked directly to Illumina...

Finally, I asked directly to Illumina tech-support, and the answer was 20-50 ng. So, I used that siRNA initial amount and it worked!
Forum: Illumina/Solexa 04-29-2011, 07:04 AM
Replies: 22
Views: 12,310
Posted By vvendr
Hi, I was actually wondering how mauch...

Hi,

I was actually wondering how mauch smallRNA I need to start, if I have smallRNA extracted without previous totalRNA isolation.
Forum: Illumina/Solexa 04-28-2011, 08:21 AM
Replies: 22
Views: 12,310
Posted By vvendr
TruSeq small RNA- sample prep

Hi,

has anyone ever tried to use small RNA as starting material? What small RNA concentration is suggest?

Thanks
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