Forum: Literature Watch
08-01-2010, 11:48 PM
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Replies: 1
Views: 2,052
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Forum: Sample Prep / Library Generation
03-22-2010, 10:31 PM
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Replies: 4
Views: 15,406
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Forum: Illumina/Solexa
01-19-2010, 05:47 PM
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Replies: 5
Views: 3,655
yes, it,s what i mean.
i read the paper...
yes, it,s what i mean.
i read the paper "Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry" of Bentley and found he use the same PE1 adapter in both single read and paired...
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Forum: Illumina/Solexa
01-19-2010, 12:34 AM
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Replies: 5
Views: 3,655
HELP：difference between SE1 and PE1 adapter
SE1 5'-ACACTCTTTCCCTACACGACC-TCTTCCGATC*T-3'
PE1 5'-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3',
and
SE2 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3'
PE2...
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Forum: Bioinformatics
12-22-2009, 05:59 PM
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Replies: 236
Views: 670,883
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Forum: Sample Prep / Library Generation
12-22-2009, 05:50 PM
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Replies: 1
Views: 2,872
help: time of adapter ligation
hello everyone , i'am sample prepation of mRNA, and i have a question about adapter ligation to the dsCDNA:
the protocol of illuminate write: incubate the sample at room temperature for 15 minutes....
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