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Forum: RNA Sequencing 05-26-2014, 07:01 PM
Replies: 1
Views: 4,393
Posted By KristenC
Paired design versus unpaired design in DESeq2

Dear all,
I am assessing differential expression between tumour and normal gland samples from the same subjects using DESeq2 and have run into a problem using the paired design.
The dataset...
Forum: Illumina/Solexa 04-15-2014, 07:13 PM
Replies: 58
Views: 41,165
Posted By KristenC
Hi Tom and Mike, Thank you for your swift...

Hi Tom and Mike,

Thank you for your swift reply.
Tom, I really like your set-up with the internal standard as a competitor, already when I first read your paper a while ago. But we were at that...
Forum: Illumina/Solexa 04-15-2014, 05:56 PM
Replies: 58
Views: 41,165
Posted By KristenC
Targeted RNA assay

Hi,

Again reviving the topic...

We are running a targeted RNA assay on our Miseq, and also have that issue with the carry-over of previously run libraries. We have two sets of about 40 targets...
Forum: RNA Sequencing 12-03-2013, 04:40 PM
Replies: 2
Views: 3,258
Posted By KristenC
No one has any ideas? I'm at loss here......

No one has any ideas?

I'm at loss here... :confused:
Forum: Literature Watch 11-26-2013, 12:21 PM
Replies: 3
Views: 5,808
Posted By KristenC
Interesting!

Hi Tom,

I really enjoyed reading your paper! You touch on a couple of very important issues present in targeted RNA-sequencing.
I am currently working on a targeted RNA-sequencing assay, and am...
Forum: RNA Sequencing 11-24-2013, 07:21 PM
Replies: 2
Views: 3,258
Posted By KristenC
DE Analysis of targeted RNA sequencing with many DE genes

Hi Everyone,
About a month ago I started working on a project involving targeted RNA seq and have been rapidly learning about the techniques/analyses,
I have however run into some theoretical...
Forum: Introductions 11-20-2013, 08:41 PM
Replies: 0
Hi!
Views: 902
Posted By KristenC
Hi!

Hi Everyone,

About a month ago I started working on a project involving targeted RNA sequencing on a Miseq/Hiseq. After numerous succesful visits to SEQanswers I finally decided to sign up and...
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