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Forum: Sample Prep / Library Generation 07-17-2018, 05:12 AM
Replies: 0
Views: 757
Posted By StephanK
Second strand generation

Hi!
I would like to use a slightly different approach for second strand synthesis. My problem is that I have an adapter sequence and that one somehow ligates to random sequences. I went over the...
Forum: Sample Prep / Library Generation 12-09-2016, 04:00 AM
Replies: 0
Views: 768
Posted By StephanK
Cel Seq2 Exonuclease and cell barcodes

Hi!

I aim to start a Cel Seq2 experiment to get transcriptome data from single cells. The paper is here: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-0938-8

There are a...
Forum: Illumina/Solexa 07-12-2016, 12:01 AM
Replies: 1
Views: 1,199
Posted By StephanK
SmartSeq2 on Illumina HiSeq 4000

Hi!

I am new to the field and I try to establish single cell transcriptome sequencing with the Fluidigm C1 machine. We already decided that we use the SmartSeq2 protocol from Clontech (SmartSeq...
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