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Forum: Sample Prep / Library Generation 06-24-2011, 04:17 AM
Replies: 3
Views: 2,164
Posted By BTS
Caliper GX

Greetings,

We're looking into purchasing a Caliper GX I or II. It's application would be mainly as a substitute to manual gel running but would also be used in some Next Gen library prep steps. ...
Forum: Illumina/Solexa 03-17-2011, 07:37 AM
Replies: 27
Views: 8,470
Posted By BTS
Is this focus issue a problem with the GAIIx? ...

Is this focus issue a problem with the GAIIx? I'm just completing a small indexing run and was unaware of the indexed PhiX. We had two lanes drop out for read two. The coincidence may be that...
Forum: Core Facilities 03-02-2011, 12:03 PM
Replies: 16
Views: 8,132
Posted By BTS
There is some very useful information here. Has...

There is some very useful information here. Has anyone used Bio-rad's Gel Doc EZ system in place of a bioanalyzer?
Forum: Sample Prep / Library Generation 02-23-2011, 11:51 AM
Replies: 11
Views: 8,653
Posted By BTS
Thanks for clarifying that Elaine. I was...

Thanks for clarifying that Elaine. I was confused when reading through the document Illumina released and called tech support about it. Definitely makes more sense. We will probably be trying to...
Forum: Sample Prep / Library Generation 02-23-2011, 08:40 AM
Replies: 11
Views: 8,653
Posted By BTS
Are you looking to index samples? TruSeq has the...

Are you looking to index samples? TruSeq has the PCR primer sequences available. Maybe this is along the lines of your question, but does anyone know why the primers are so long and include the...
Forum: Sample Prep / Library Generation 02-23-2011, 07:46 AM
Replies: 19
Views: 10,734
Posted By BTS
Does anyone have any information one the...

Does anyone have any information one the performance of Pippin vs. Caliper XT? We are currently looking into the two options and are on the fence. I just got off the phone with a Caliper rep and...
Forum: Sample Prep / Library Generation 02-15-2011, 06:10 AM
Replies: 8
Views: 4,240
Posted By BTS
We are going to be sequencing on a GAIIx with...

We are going to be sequencing on a GAIIx with 2x75 bp reads. Wouldn't we still have to fragment to remove bias towards the ends of the fragments?

I don't have the exact pricing but from what I...
Forum: Sample Prep / Library Generation 02-10-2011, 12:58 PM
Replies: 8
Views: 4,798
Posted By BTS
I was eluting with two washes of 30 uL in a...

I was eluting with two washes of 30 uL in a QiaQuick column and diluting to a final volume of 100 uL. I read similar information about the columns as what you posted and have changed my protocol to...
Forum: Sample Prep / Library Generation 02-10-2011, 09:07 AM
Replies: 8
Views: 4,240
Posted By BTS
Halo Genomics Selector Technology

I'm wondering if anyone has experience with Halo Genomics' Selector Technology (formerly O-link Genomics)? We are considering this technology for targeted resequencing of a panel of about 500-600...
Forum: Illumina/Solexa 02-03-2011, 06:29 AM
Replies: 45
Views: 23,032
Posted By BTS
Do you collect multiple times in a single E-gel...

Do you collect multiple times in a single E-gel run? We have tried collected at three different points (collect, run gel 30 seconds, collect...). Also, where does your collection point for 380 bp...
Forum: Sample Prep / Library Generation 02-03-2011, 06:21 AM
Replies: 8
Views: 4,798
Posted By BTS
Oops, I forgot to include that. We quantified...

Oops, I forgot to include that. We quantified our DNA via Qubit Flourometer prior to fragmenting AND after.
Forum: Sample Prep / Library Generation 02-03-2011, 05:58 AM
Replies: 8
Views: 4,798
Posted By BTS
NEB Fragmentase

We are trying to fragment genomic DNA using NEB's Fragmentase enzyme with a 20-23 minute incubation. Our goal is to reach fragments near 300bp for sequencing on GAIIx. Has anyone used this enzyme...
Forum: Illumina/Solexa 01-28-2011, 05:09 AM
Replies: 45
Views: 23,032
Posted By BTS
Thank you both for the information. Since this...

Thank you both for the information. Since this is a first attempt with the kit and we want to make sure that everything is done right we are going to follow the recommendations to use SyBr gold and...
Forum: Illumina/Solexa 01-27-2011, 08:04 AM
Replies: 45
Views: 23,032
Posted By BTS
Help with TruSeq gDNA library prep

We are currently working through the TruSeq protocol to prep gDNA for hybridization to Nimblegen probes and PE sequencing on a GAIIx... Our problem is that we attempted using Invitrogen's eGels for...
Forum: Illumina/Solexa 11-10-2010, 08:57 AM
Replies: 1
Views: 865
Posted By BTS
Problem with Amplifcation Manifolds

We have recently been experiencing problems with the amplifcation manifolds from Illumina in their ability to form a tight seal on the cluster station. The result has been decreased reagent flow and...
Forum: Illumina/Solexa 10-12-2010, 05:17 AM
Replies: 16
Views: 7,800
Posted By BTS
Thanks for the info advanT!

Thanks for the info advanT!
Forum: Illumina/Solexa 10-05-2010, 01:00 PM
Replies: 16
Views: 7,800
Posted By BTS
I was recently forwarded a link about Bioo...

I was recently forwarded a link about Bioo Scientific.

http://www.newswiretoday.com/news/78473/

It looks like their kits are even cheaper than NEB's. Does anyone know anything about these...
Forum: Illumina/Solexa 09-21-2010, 01:18 PM
Replies: 18
Views: 5,747
Posted By BTS
Bridge amplification and fragment size

Hi All,
I'm new to this whole NGS business. I will be using an Illumina GAII. In a recent article from Bio Techniques, I read that longer (about 600bp) fragments can increase the uniformity of...
Forum: Illumina/Solexa 09-16-2010, 11:16 AM
Replies: 73
Views: 351,230
Posted By BTS
Bridge Amplification

Hi All,
I'm new to this whole NGS business. I will be using an Illumina GAII. In a recent article from Bio Techniques, I read that longer (about 600bp) fragments can increase the uniformity of...
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