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Forum: Sample Prep / Library Generation 05-03-2012, 09:21 AM
Replies: 13
Views: 23,033
Posted By kainsteven
Thank you for the question. As mechanical or...

Thank you for the question. As mechanical or enzymatic fragmentation methods will produce a distribution of fragment sizes we typically choose a median size of 350 to 400 bp for 2 x 90 bp reads....
Forum: Illumina/Solexa 11-20-2010, 09:56 AM
Replies: 24
Views: 11,209
Posted By kainsteven
The Ovation WGA FFPE System from NuGEN can be...

The Ovation WGA FFPE System from NuGEN can be used for DNA from FFPE or Fresh Frozen (FF) material with input amounts as low as 100 ng.

Steve
Forum: Introductions 11-08-2010, 08:50 AM
Replies: 6
Views: 3,526
Posted By kainsteven
Hi Anand, Can you please provide some...

Hi Anand,

Can you please provide some details regarding the impact of OCT treatment on the samples? Ovation RNA-Seq FFPE is preferable if you have degraded samples, and/or cross-linking typically...
Forum: RNA Sequencing 10-15-2010, 05:04 PM
Replies: 1
Views: 2,162
Posted By kainsteven
Ovation RNA-Seq System

As I've posted in prior threads, the Pico System from NuGEN (Ovation Pico WTA System) was not designed for sequencing applications, and was optimized for production of single-stranded cDNA used with...
Forum: Literature Watch 09-13-2010, 10:12 AM
Replies: 2
Views: 4,739
Posted By kainsteven
NuGEN's RNA-Seq solutions

Newsbot!,

Thank you for posting the abstract of this paper comparing the use of two of NuGEN's amplification systems. I wanted to point out that this was a microarray study, and did not include...
Forum: Sample Prep / Library Generation 07-30-2010, 10:21 AM
Replies: 15
Views: 5,843
Posted By kainsteven
Ovation RNA-Seq System

Hi Staoudi,

In answer to your questions:

1. Yes, our system is compatible with purified mRNA. We have not characterized the input amounts for mRNA, as our system was validated for total RNA in...
Forum: Introductions 06-26-2010, 08:00 PM
Replies: 6
Views: 3,526
Posted By kainsteven
Yes, compatible with SOLiD and 454

The product from the Ovation RNA-Seq workflow is ds cDNA, and hence compatible with any library prep system using this starting material. We have several customers that have used the system...
Forum: Illumina/Solexa 06-25-2010, 09:57 AM
Replies: 5
Views: 4,661
Posted By kainsteven
Ovation RNA-Seq, 3'-DGE and Encore NGS Library Systems

It is no longer necessary to use the exon module to produce the double-stranded cDNA. We have packaged the complete solution for producing a dscDNA starting with total RNA in either an RNA-Seq or...
Forum: Illumina/Solexa 06-10-2010, 09:32 AM
Replies: 5
Views: 4,661
Posted By kainsteven
Users of the Ovation RNA-Seq System

We have several conference posters and webinars posted on our web site to illustrate studies with low inputs of total RNA using the Ovation RNA-Seq System:...
Forum: RNA Sequencing 06-10-2010, 09:26 AM
Replies: 7
Views: 6,136
Posted By kainsteven
Users of Ovation RNA-Seq

We have had several users generate good results using low input amounts of total RNA with the Ovation RNA-Seq System. If you are interested, we have several customer posters and webinars from recent...
Forum: Sample Prep / Library Generation 03-02-2010, 05:02 PM
Replies: 2
Views: 3,026
Posted By kainsteven
RNA-Seq from 500 pg total RNA

We recently presented results at AGBT showing the use of our Ovation RNA-Seq System for low input studies such as you mention here. The input range for total RNA is from 500 pg to 10 ng, with no...
Forum: The Pipeline 03-01-2010, 06:40 AM
Replies: 10
Views: 7,009
Posted By kainsteven
Great presentation at AGBT, looking forward to...

Great presentation at AGBT, looking forward to the Ion Torrent story as it emerges over the next few months.

Steve
Forum: Introductions 02-21-2010, 10:18 PM
Replies: 2
Views: 1,709
Posted By kainsteven
Can't wait to learn more about your technology...

Can't wait to learn more about your technology and products!
Forum: Sample Prep / Library Generation 01-13-2010, 02:52 PM
Replies: 13
Views: 23,033
Posted By kainsteven
For RNA-Seq applications, we have seen better...

For RNA-Seq applications, we have seen better results by excising material corresponding to the peak of the bioanalyzer trace for library preparation. In most cases, this corresponds to ~ 250 – 350...
Forum: Sample Prep / Library Generation 01-13-2010, 02:43 PM
Replies: 15
Views: 5,843
Posted By kainsteven
We have recently introduced a kit that requires...

We have recently introduced a kit that requires as little as 500 pg of total RNA input, and produces ds cDNA compatible with the GAIIx workflow. Information about the product, including the User...
Forum: Introductions 12-06-2009, 11:15 AM
Replies: 6
Views: 3,526
Posted By kainsteven
Hello from NuGEN Technologies

Steve Kain here. We have recently introduced a solution for RNA-Seq sample prep with inputs of total RNA in the range of 500 pg to 100 ng. The product is double-stranded cDNA in the size range of...
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