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Forum: Sample Prep / Library Generation 11-04-2013, 10:32 AM
Replies: 3
Views: 3,959
Posted By mgravina
Double-SPRI size selection

We are attempting to automate double-SPRI size selection, in which two successive SPRI-bead-based size cuts are used to select DNA approximately between 200 and 450 bp in length. We are having a very...
Forum: Illumina/Solexa 07-22-2013, 08:29 AM
Replies: 0
Views: 1,277
Posted By mgravina
Regenerating CIFs (Cluster Intensity Files)

Hi all. My lab ran a GAIIx flowcell with run parameters set to save and copy images but not Cluster Intensity Files. We really need those CIFs for a custom analysis.

We would try Off-Line...
Forum: Illumina/Solexa 03-27-2012, 08:28 AM
Replies: 0
Views: 1,599
Posted By mgravina
Custom recipe for GAII: one lane at a time

Hello all. I'm trying to determine whether it is possible to edit a GAII recipe so that imaging is carried out on a limited number of lanes instead of the whole flowcell. I believe it should be...
Forum: Illumina/Solexa 01-17-2012, 08:45 AM
Replies: 0
Views: 1,197
Posted By mgravina
Ambient Temperature initialization error

While initializing the Illumina data collection software, I receive the following error:

File: "C:\Illumina\SCS2.9\DataCollection\bin\release\HyperKastor\FlexTejAMBTmpr.dll": Error in the...
Forum: Illumina/Solexa 11-17-2011, 09:23 AM
Replies: 12
Views: 4,211
Posted By mgravina
Having similar problems

Our laboratory has been having problems that sound suspiciously similar to yours. Here's the link to the thread:
http://seqanswers.com/forums/showthread.php?p=57156&posted=1#post57156

I see that...
Forum: Illumina/Solexa 11-17-2011, 09:09 AM
Replies: 2
Views: 3,322
Posted By mgravina
Thanks for the links, they're very informative!...

Thanks for the links, they're very informative! In response to your suggestions of possible causes:

1. We've seen the ectopic annealing peak plenty of times before. These peaks seem to be...
Forum: Illumina/Solexa 11-17-2011, 08:17 AM
Replies: 2
Views: 3,322
Posted By mgravina
Size-selection problem with ChIPseq-prep on Beckman SPRIworks

Our core lab has been seeing unusual failures of size-selection when we prep ChIP samples for Illumina sequencing on our Beckman SPRI-TE robot. We generally size-select for 200-400bp. However, since...
Forum: General 05-25-2011, 11:16 AM
Replies: 3
Views: 1,830
Posted By mgravina
Thanks for the info!

Thanks for the info!
Forum: General 05-24-2011, 11:12 AM
Replies: 3
Views: 1,830
Posted By mgravina
5' versus 3' barcoding

Hello all. Can anyone offer insight into the pros and cons of placing index barcodes at the 5' versus the 3' end of the read?
Forum: Illumina/Solexa 02-17-2011, 10:35 AM
Replies: 0
Views: 1,908
Posted By mgravina
Washed out flowcell tiles- overclustered? Optical error?

Hi all. I'm having a problem on a GAIIx where the tiles for several lanes appear "washed out" from one side. There is a big, white blur coming off the left-hand edge of the tiles, obscuring about...
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