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Forum: Bioinformatics 09-12-2017, 08:00 AM
Replies: 7
Views: 2,767
Posted By GenoMax
Not what I was imagining then. If the...

Not what I was imagining then. If the tags/extraneous sequence present is not interfering with your OTU assignments then you could do the trimming/assembly later.

Are you at least merging the...
Forum: Bioinformatics 09-12-2017, 07:45 AM
Replies: 7
Views: 2,767
Posted By GenoMax
I assume your minibarcodes are tagging species...

I assume your minibarcodes are tagging species specific primers that allow you to bin the reads first. So once you bin the three products for each species you would trim the barcodes/merge the reads...
Forum: Bioinformatics 09-04-2017, 10:59 AM
Replies: 7
Views: 2,767
Posted By GenoMax
Provided your primers have covered the gene fully...

Provided your primers have covered the gene fully you could assemble the reads (after merging the pairs post demultiplexing/removal of barcodes).

Should be easy enough to test. Use bbmerge.sh...
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