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Forum: De novo discovery 09-10-2016, 10:44 PM
Replies: 3
Views: 8,282
Posted By sebl
These are isolate libraries. When I BLAST...

These are isolate libraries.

When I BLAST short contigs (<200bp) with high coverage (10'sx), these are often parts of ribosomal genes. When I BLAST contigs of length 200-~500bp, where most of low...
Forum: De novo discovery 09-10-2016, 12:54 AM
Replies: 3
Views: 8,282
Posted By sebl
Minimum contig coverage to keep

Dear all,

I am working on de novo assembly of bacterial genomes. Genomes were sequenced with Miseq 2x250 libs with an average coverage ~40X. Adapters were trimmed with cutadapt by the seq...
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