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Forum: Sanger/Dye Terminator 06-19-2015, 07:55 AM
Replies: 4
Views: 6,675
Posted By Jessica_L
To be clear-- your initial experiments isolated...

To be clear-- your initial experiments isolated mRNA from cells, which you reverse transcribed into cDNA and sanger sequenced. Now, for pyrosequencing, you tried isolating DNA straight from the cells...
Forum: Sanger/Dye Terminator 06-18-2015, 07:56 AM
Replies: 4
Views: 6,675
Posted By Jessica_L
Without knowing more about what you're doing, or...

Without knowing more about what you're doing, or what kind of troubleshooting you've already done, it's hard to say why your other methodologies aren't working. Since all you've said so far is that a...
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