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Forum: Bioinformatics 09-12-2017, 11:37 AM
Replies: 7
Views: 2,767
Posted By lvogel
OK, thanks. I merge the paired-ends as a first...

OK, thanks. I merge the paired-ends as a first step, yes, if that's what you mean.
Forum: Bioinformatics 09-12-2017, 07:54 AM
Replies: 7
Views: 2,767
Posted By lvogel
Our primers aren't species-specific. We use the...

Our primers aren't species-specific. We use the same 3 primer sets for all of the mixed samples of insects. We don't know what the species are likely to be until BLASTing at the end.
Forum: Bioinformatics 09-12-2017, 07:38 AM
Replies: 7
Views: 2,767
Posted By lvogel
Dear GenoMax (or anyone), Is there a reason why...

Dear GenoMax (or anyone),
Is there a reason why assembly should be done at the step where you said (after PE merging and removal of primers/tags), as opposed to waiting until the end, when we have...
Forum: Bioinformatics 09-05-2017, 12:57 AM
Replies: 7
Views: 2,767
Posted By lvogel
Thumbs up Thanks

Thanks for the reply. Sounds good. I'll give it a try, and post again if I have problems.
Forum: Bioinformatics 09-04-2017, 07:34 AM
Replies: 7
Views: 2,767
Posted By lvogel
Question Assembling mini barcodes into full-length gene

Hi,
I have metabarcoding reads from an Illumina MiSeq run. I usually use a primer set which amplifies a certain fragment of COI. Now, however, we have used three different primer sets which produce...
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