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Forum: Sanger/Dye Terminator 06-29-2015, 04:52 AM
Replies: 4
Views: 6,675
Posted By Anke
Thank you very much for all these suggestions and...

Thank you very much for all these suggestions and ideas. the problem is that I am really not having all these informations for everything was done in cooperation with people that should be experts in...
Forum: Sanger/Dye Terminator 06-18-2015, 08:26 AM
Replies: 4
Views: 6,675
Posted By Anke
hi, thx for answering - The sequence is indeed...

hi, thx for answering
- The sequence is indeed having a high GC content(>85%) however I am using betaine and so far that worked quite well.(DMSO is not helping it rather makes things worse)
- I...
Forum: Sanger/Dye Terminator 06-18-2015, 04:46 AM
Replies: 4
Views: 6,675
Posted By Anke
My Sanger results can not be reproduced by other seq methods...?!

Hi everyone,

I am having troubles with proofing the results I obtained in Sanger sequencing by other methods.
At the beginning of my PhD I isolated RNA of human cancer cells, transcribed it into...
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