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Forum: Sample Prep / Library Generation 11-06-2015, 11:24 AM
Replies: 9
Views: 2,236
Posted By ATϟGC
You are correct nucacidhunter that the theory...

You are correct nucacidhunter that the theory does not always hold true. I apologize that my second sentence did not clearly convey that it is an approximation based on the assumption that base order...
Forum: Sample Prep / Library Generation 11-06-2015, 06:47 AM
Replies: 9
Views: 2,236
Posted By ATϟGC
In theory, both BamHI (GGATCC) and PstI (CTGCAG)...

In theory, both BamHI (GGATCC) and PstI (CTGCAG) should cut with equal frequency in any given genome as they both have a 6bp recognition sequence with the same frequency of A-T and G-C base pairs...
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