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Forum: Oxford Nanopore 07-24-2017, 07:18 AM
Replies: 11
Views: 4,959
Posted By VinceM
Yes, you would lose all your quality data.

Yes, you would lose all your quality data.
Forum: Oxford Nanopore 07-24-2017, 06:38 AM
Replies: 11
Views: 4,959
Posted By VinceM
Just one more suggestion. Copy a small part of...

Just one more suggestion. Copy a small part of the fastq file (for example, 50 - 100 sequences) and run that truncated file through the analysis programs.
Forum: Oxford Nanopore 07-24-2017, 06:05 AM
Replies: 11
Views: 4,959
Posted By VinceM
Certainly looks OK. Have you tried converting the...

Certainly looks OK. Have you tried converting the fastq to fasta to see if that would work? If it's a memory issue that might help.

Not sure I can contribute much more - wet lab person, not...
Forum: Oxford Nanopore 07-24-2017, 05:41 AM
Replies: 11
Views: 4,959
Posted By VinceM
Hi 7tbear7, I'm surprised you are having...

Hi 7tbear7,

I'm surprised you are having trouble with fastq files. Fast5 files were a nightmare to convert to fastq when I first started using the MinION; once in fastq they were relatively easy...
Forum: Oxford Nanopore 07-06-2017, 05:53 AM
Replies: 2
Views: 3,308
Posted By VinceM
Someone in your organisation must have registered...

Someone in your organisation must have registered with Nanopore before they could purchase the MinION and flow cells. They will be able to add members to the existing team, and that will allow them...
Forum: Bioinformatics 08-02-2016, 06:28 AM
Replies: 2
Views: 1,386
Posted By VinceM
Fairly sure that (T) stands for type strain - if...

Fairly sure that (T) stands for type strain - if you filter, selecting only type strains, all you see are sequences with a (T) subscript.
Forum: Introductions 03-16-2016, 08:27 AM
Replies: 0
Views: 1,002
Posted By VinceM
Hello

Hi,

I'm from a well established molecular biology lab in an government agricultural institution. We use PCR, real-time PCR, digital droplet PCR, SSR fingerprinting and Sanger-based sequencing....
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