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Forum: Illumina/Solexa 11-21-2019, 05:22 AM
Replies: 7
Views: 5,354
Posted By Ross_Mcf
Hi @Bukowski, thanks for your feedback. ...

Hi @Bukowski, thanks for your feedback.

While I fully agree regarding the 15% PhiX and low diversity library I was more concerned with the statement from the protocol about the indices. I will be...
Forum: Illumina/Solexa 11-21-2019, 05:09 AM
Replies: 7
Views: 5,354
Posted By Ross_Mcf
Thanks for the answer @sifekonja! I...

Thanks for the answer @sifekonja!

I initially thought the failure might be due to low library or barcode diversity. This is copied from the library prep protocol: "The Illumina sequencers will...
Forum: Illumina/Solexa 11-20-2019, 11:59 PM
Replies: 7
Views: 5,354
Posted By Ross_Mcf
MiSeq - issue with index and read 2 (Low Q30)

I recently had a MiSeq run fail and am not 100% sure what caused it.

Here are some details:
Amplicon sequencing run of RNA FFPE samples (125 genes) using a MiSeq V3 (600 cycle) kit loaded at 14...
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