Forum: Oxford Nanopore
Yesterday, 02:42 AM
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Replies: 1
Views: 579
Well, there's the official ONT protocol which...
Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol...
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Forum: Oxford Nanopore
Yesterday, 02:37 AM
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Replies: 1
Views: 606
I assume you mean Mk1b; the Mk1c is a single...
I assume you mean Mk1b; the Mk1c is a single enclosed unit, including sequencer, screen, and computer.
There are two must-haves for a MinION sequencing computer: an NVIDIA GPU graphics card, and...
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Forum: Sample Prep / Library Generation
04-14-2022, 04:17 AM
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Replies: 5
Views: 915
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Forum: RNA Sequencing
04-11-2022, 12:18 PM
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Replies: 1
Views: 1,031
You haven't given enough information about the...
You haven't given enough information about the NovaSeq runs. What chip? What read length? Paired end?
Are you paying the same amount for ONT as for NovaSeq? If so, you'd likely be using PromethION...
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Forum: Sample Prep / Library Generation
04-11-2022, 12:05 PM
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Replies: 5
Views: 915
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Forum: Oxford Nanopore
01-31-2022, 01:39 PM
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Replies: 2
Views: 4,313
All the platforms you mentioned use cluster...
All the platforms you mentioned use cluster consensus to improve single-read accuracy. This can also be done on nanopore by using more reads.
Also, the long read lengths can compensate for an...
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Forum: Oxford Nanopore
01-18-2022, 06:32 PM
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Replies: 1
Views: 4,104
Try needle-shearing your DNA prior to doing the...
Try needle-shearing your DNA prior to doing the CRISPR-Cas9 digest, e.g. 20 passes with a 26G or 29G needle (see here (http://www.longreadclub.org/mountain-protocol/) for more information). This has...
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Forum: Oxford Nanopore
01-18-2022, 06:23 PM
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Replies: 2
Views: 1,874
Also, the cDNA kits are a stranded sequencing...
Also, the cDNA kits are a stranded sequencing process (different adapter sequences for forward and reverse); you'd lose that benefit by using either the ligation kit or rapid kit on pre-prepared cDNA.
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Forum: Bioinformatics
01-21-2020, 04:33 PM
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Replies: 1
Views: 1,434
No. Those p-values usually apply to the...
No. Those p-values usually apply to the confidence in the value of the distinguishing statistic (a statistic which, as it happens, is frequently absent from abstracts and the non-supplementary paper...
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Forum: Bioinformatics
01-21-2020, 04:25 PM
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Replies: 2
Views: 8,392
Have a look at the bcftools...
Have a look at the bcftools (https://samtools.github.io/bcftools/bcftools.html) package. It supports uncompressed and gzip-compressed VCF file formats as input, as well as the BCF format, and...
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Forum: Ion Torrent
08-11-2019, 01:01 AM
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Replies: 19
Views: 14,572
Hobby Money
Customers don't pay return shipping to the UK. That cost is met by ONT, even for flow cells that are being returned from New Zealand. I suppose if you want to be cynical, you could say the return...
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Forum: Bioinformatics
08-08-2019, 03:58 PM
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Replies: 10
Views: 2,701
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Forum: Ion Torrent
08-08-2019, 03:50 PM
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Replies: 19
Views: 14,572
The MinION flow cell return to ONT is so that...
The MinION flow cell return to ONT is so that they can recycle them for use in training and internal research. If you contaminate the flow cells such that they are not safe for posting back, you...
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Forum: Oxford Nanopore
05-10-2019, 01:57 AM
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Replies: 6
Views: 13,551
External access
On the other side of things, a MinION sequencing run won't work unless the computer has access to the outside via non-standard ports, which are used to fetch firmware, and send telemetry data a short...
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Forum: Illumina/Solexa
04-07-2019, 03:34 PM
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Replies: 3
Views: 1,382
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Forum: Oxford Nanopore
04-07-2019, 03:26 PM
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Replies: 2
Views: 8,395
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Forum: Oxford Nanopore
04-07-2019, 03:18 PM
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Replies: 2
Views: 6,922
Just buy new flow cells. Anything from 2017 is...
Just buy new flow cells. Anything from 2017 is likely to be dead, and you'll get more sequence in your first hour with new flow cells than you would have got in 48 hours from working flow cells in...
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Forum: Oxford Nanopore
04-07-2019, 03:12 PM
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Replies: 1
Views: 8,373
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Forum: Oxford Nanopore
04-07-2019, 03:07 PM
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Replies: 1
Views: 6,855
As far as I'm aware, it doesn't matter....
As far as I'm aware, it doesn't matter. Nanopolish uses the called fastq files together with the signal in the fast5 files, so both should work. I expect the main difference between the two fast5...
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Forum: Oxford Nanopore
04-07-2019, 02:55 PM
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Replies: 6
Views: 13,551
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Forum: Oxford Nanopore
04-07-2019, 02:48 PM
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Replies: 2
Views: 7,604
Qubit or Quantus (or other similar...
Qubit or Quantus (or other similar fluorescence-based methods) are pretty much essential for nanopore sequencing. The nanodrop has issues with RNA contamination, and gives concentrations that can be...
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Forum: Oxford Nanopore
04-07-2019, 02:43 PM
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Replies: 2
Views: 6,813
The Starter Pack is not sufficient for doing...
The Starter Pack is not sufficient for doing multiple samples. In ONT's infinite wisdom, they decided to put the rapid kit as an option, rather than the rapid barcoding kit (despite them having...
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Forum: General
02-11-2019, 09:59 PM
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Replies: 1
Views: 3,589
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Forum: Oxford Nanopore
10-19-2018, 03:43 PM
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Replies: 0
Views: 7,223
Flongle flow cells - now shipping
Oxford Nanopore Technologies have started shipping Flongle adapters and flow cells to customers. These are disposable flow cells using fewer pores and a cheaper design ($5000 USD for 48 flow cells)....
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Forum: Oxford Nanopore
09-28-2018, 03:42 AM
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Replies: 18
Views: 15,190
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