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Forum: Vendor Forum 07-22-2013, 06:37 AM
Replies: 0
Views: 1,237
Posted By epibio
Something new is Budding…

Now Available from Epicentre (an Illumina company): The ScriptSeq™ Complete Kit (Yeast) (http://www.epibio.com/applications/rna-sequencing/rna-library-prep/scriptseq-complete-gold-kit-(yeast))...
Forum: Illumina/Solexa 07-17-2013, 09:10 AM
Replies: 18
Views: 9,103
Posted By epibio
The ScriptMiner Kit includes tobacco Acid...

The ScriptMiner Kit includes tobacco Acid Pyrophosphatase (TAP). TAP will convert 5'-capped and 5'-tripphosphate ends to 5'-monophosphate for subsequent 5'-adaptor ligation. the TAP step is...
Forum: Vendor Forum 03-20-2013, 10:16 AM
Replies: 0
Views: 1,767
Posted By epibio
RNA-Seq sample prep from blood

The ScriptSeq™ Complete Gold Kit (Blood) (http://www.epibio.com/products/rna-sequencing/rna-library-prep/scriptseq-complete-gold-kit-(blood)) from Epicentre generates high complexity RNA-Seq...
Forum: Vendor Forum 02-18-2013, 07:20 AM
Replies: 0
Views: 2,287
Posted By epibio
Globin & rRNA removal from blood RNA

The new Globin-Zero™ Gold Kit (http://www.epibio.com/products/rna-sequencing/rrna-removal/globin-zero-gold-kit)from Epicentre effectively removes the highly abundant adult globin mRNAs and the...
Forum: Vendor Forum 12-12-2012, 01:24 PM
Replies: 0
Views: 8,533
Posted By epibio
New Ribo-Zero™ Kit (Epidemiology):rRNA removal from microbial-infected mammalian RNA

RNA-Seq based host/microbe disease and metatranscriptomic studies just got easier with Epicentre’s new Ribo-Zero Magnetic Gold Kit (Epidemiology) (http://www.epibio.com/item.asp?ID=604). The kit...
Forum: Industry Jobs! 07-30-2012, 09:52 AM
Replies: 0
Views: 2,021
Posted By epibio
Associate Scientist (R&D) at Epicentre: Madison, WI

Epicentre (an Illumina company) is hiring an Associate Scientist (R&D) at its facility in Madison, WI. The successful candidate will execute and interpret molecular biology experiments as well as...
Forum: Vendor Forum 07-11-2012, 11:30 AM
Replies: 0
Views: 1,814
Posted By epibio
New ScriptSeq Complete Kits: end-to-end RNA-Seq solution

Epicentre’s ScriptSeq™ Complete (http://www.epibio.com/item.asp?ID=599) Kits are now available. The kits combine Epicentre's industry-leading Ribo-Zero™ rRNA removal technology and rapid ScriptSeq v2...
Forum: Industry Jobs! 05-07-2012, 01:17 PM
Replies: 0
Views: 2,058
Posted By epibio
Bioinformatics Scientist 2 at Epicentre (Madison, WI)

Epicentre (an Illumina company) is seeking an exceptional individual to contribute to technical comparative analysis of genomic data sets and related software tools. For more information and to...
Forum: Sample Prep / Library Generation 05-07-2012, 10:41 AM
Replies: 22
Views: 11,651
Posted By epibio
We typically see >99.9% removal of 5.8S and...

We typically see >99.9% removal of 5.8S and >97.8% of 5S rRNAs in partially degraded RNA samples. Depending on the RIN of the original sample, I'd be concerned about over-fragmentation during library...
Forum: Vendor Forum 04-06-2012, 06:05 AM
Replies: 10
Views: 6,253
Posted By epibio
The ScriptSeq v2 kit will perform well with 1 ng...

The ScriptSeq v2 kit will perform well with 1 ng total RNA; as you expect, the majority of your reads would be ribosomal RNA.
Forum: Vendor Forum 03-22-2012, 06:03 AM
Replies: 0
Views: 1,131
Posted By epibio
Message for user "genomcc"

I'm unable to reply to your PM, since your account is not accepting messages. Please contact me using the direct e-mail link on my profile, regarding your question about Ribo-Zero kits.

Thanks!
Forum: Vendor Forum 03-16-2012, 06:47 AM
Replies: 10
Views: 6,253
Posted By epibio
In theory, you could use the kit with total RNA...

In theory, you could use the kit with total RNA at the higher end of the input range. However, since 95-99% of your reads will map to ribosomal RNA, it would not be a good idea.
Forum: Vendor Forum 03-14-2012, 12:28 PM
Replies: 0
Views: 2,236
Posted By epibio
Improved ScriptMiner small-RNA-Seq kit now available

Epicentre's ScriptMiner Kit (http://www.epibio.com/item.asp?ID=581) has been improved with a streamlined protocol that requires less hands-on time. The ScriptMiner Kit produces nonbarcoded or...
Forum: Vendor Forum 03-13-2012, 10:59 AM
Replies: 36
Views: 9,217
Posted By epibio
Yes, that would work for SOLiD.

Yes, that would work for SOLiD.
Forum: Vendor Forum 03-13-2012, 06:10 AM
Replies: 36
Views: 9,217
Posted By epibio
Especially with an international shipment, there...

Especially with an international shipment, there could be issues with shipping and storage that may cause problems, which is why I suggested more extensive troubleshooting with tech support. If there...
Forum: Vendor Forum 03-12-2012, 07:54 AM
Replies: 36
Views: 9,217
Posted By epibio
You're right that the ScriptSeq method will not...

You're right that the ScriptSeq method will not be compatible with SOLiD.

If you are having yield problems with a Ribo-Zero kit, I would be concerned that the same problems would occur if you use...
Forum: Vendor Forum 03-12-2012, 04:28 AM
Replies: 36
Views: 9,217
Posted By epibio
Considering that rRNA constituted 99% of your...

Considering that rRNA constituted 99% of your sample, 50-70 ng of rRNA-depleted RNA is in line with the expected yield. This would be sufficient to prepare an RNA-Seq library. For example, our...
Forum: Sample Prep / Library Generation 02-08-2012, 02:38 PM
Replies: 22
Views: 11,651
Posted By epibio
Can your provide more information about: i) the...

Can your provide more information about: i) the source and quality of the total RNA; ii) how much rRNA-depleted RNA you used as input for library prep; iii) the library prep method, including...
Forum: Vendor Forum 02-06-2012, 06:48 AM
Replies: 1
Views: 1,920
Posted By epibio
Update: Ribo-Zero Kits are now available...

Update: Ribo-Zero Kits are now available (http://www.epibio.com/ribozero/ribozero.asp) in the new, magnetic format for animal, plant, and bacterial RNA samples.
Forum: Sample Prep / Library Generation 02-02-2012, 08:27 AM
Replies: 22
Views: 11,651
Posted By epibio
It depends on your library prep method (for...

It depends on your library prep method (for example, fragmentation conditions). With our ScriptSeq library prep, we typically get a range of 200-1,000 bp with a peak around 350 bp. You would not...
Forum: Sample Prep / Library Generation 01-11-2012, 07:08 AM
Replies: 22
Views: 11,651
Posted By epibio
Yes, we have two types...

Yes, we have two types (http://www.epibio.com/item.asp?ID=585) of plant Ribo-Zero kits (for RNA from seeds or roots/leaves). We will be presenting a poster...
Forum: Vendor Forum 01-11-2012, 05:44 AM
Replies: 1
Views: 1,920
Posted By epibio
Ribo-Zero (Human/Mouse/Rat) kit now in magnetic format

Epicentre's most popular Ribo-Zero Kit (http://www.epibio.com/item.asp?ID=576) is now available in a convenient magnetic format. Ribo-Zero kits remove >99% of rRNA from intact or degraded total RNA...
Forum: Vendor Forum 12-20-2011, 01:46 PM
Replies: 10
Views: 6,253
Posted By epibio
This is the 600-gene coverage, single-end...

This is the 600-gene coverage, single-end sequencing reads from the 5' end so there is an expected drop in coverage at the 3' end. I'll post the UCSC browser plots when they are available.
...
Forum: Vendor Forum 12-20-2011, 10:28 AM
Replies: 10
Views: 6,253
Posted By epibio
Not yet, but we're working on it. At present, the...

Not yet, but we're working on it. At present, the other data we have is a 600-gene coverage plot that I can send you.
Forum: Illumina/Solexa 12-15-2011, 07:43 AM
Replies: 4
Views: 3,663
Posted By epibio
Mammalian cultured cell lines should work well...

Mammalian cultured cell lines should work well with the Ribo-Zero H/M/R kit. For other types of samples, please see our species compatibility (http://www.epibio.com/ribozero/ribozero.asp) list
Showing results 1 to 25 of 89

 


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