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Forum: General 09-18-2019, 08:50 AM
Replies: 0
Views: 544
Posted By krutipedali
Commercial vs Self-ordered Index primers

Hello Everybody!

Could anyone please explain to me why should we buy commercial indexing primers ? Are they purified somehow specifically ? Why the amounts IDT and Illumina sell are so small ?
...
Forum: Sample Prep / Library Generation 02-28-2017, 04:16 AM
Replies: 2
Views: 2,602
Posted By krutipedali
No, those libraries haven`t been sent for...

No, those libraries haven`t been sent for sequencing. Instead we have produced another samples. The thing is they are the way too overtagmented, so go down with incubation time(even to 5-10 minutes)...
Forum: Sample Prep / Library Generation 12-27-2016, 02:52 AM
Replies: 2
Views: 2,602
Posted By krutipedali
On-Plate ATAC-seq

Hello,

Could anyone please drop any comments on libraries that i have prepared using on-plate ATAC protocol (DOI: 10.1186/s13059-015-0840-9). Libraries seem to be overtagmented, but i would like...
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