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Forum: Bioinformatics 05-24-2018, 12:08 PM
Replies: 1
Views: 1,101
Posted By lfaller
Error encountered when running kmercountexact.sh from bbmap

Hi all,

I am encountering a java.lang.AssertionError when trying to run kmercountexact.sh.

The two input files are 820 Mb each. I also tried running this script with subsetted data of the...
Forum: Illumina/Solexa 07-26-2017, 07:49 AM
Replies: 3
Views: 3,270
Posted By lfaller
Thank you for suggestion! I went back to...

Thank you for suggestion!

I went back to the lab tech and asked her to take a critical look at the indices. She regenerated the file using the Illumina Experiment Manager and now it worked. I...
Forum: Illumina/Solexa 07-11-2017, 08:45 AM
Replies: 3
Views: 3,270
Posted By lfaller
Question Advice needed for --use-bases-mask option (bcl2fastq)

Hi all,

A colleague in the lab asked me to demultiplex a recent NextSeq run. She loaded it with samples prepared from two libraries. One library had single indices and one had dual indices. She...
Forum: Introductions 07-11-2017, 08:25 AM
Replies: 0
Views: 754
Posted By lfaller
Smile Hello from Boston

Hello everyone,

I've been working in the bioinformatics field for a few years now but the learning never stops. I'm particularly interested in the biology of metagenomics and microbial communities...
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