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Forum: 454 Pyrosequencing 06-22-2015, 04:38 AM
Replies: 11
Views: 4,434
Posted By mps4208
The Agilent will not show the small reads which...

The Agilent will not show the small reads which is frustrating, the only way to see them ahead of time is to actually do the 454 PCR "Sample Library Quality Control" in Tech bull #2011-002. I've...
Forum: Academic/Non-Profit Jobs 06-01-2015, 09:40 AM
Replies: 0
Views: 421
Posted By mps4208
Research Assistant position-lab bench only

If you have experience with next-generation sequencing and looking for a position in North Carolina, please follow link to apply.

https://unc.peopleadmin.com/postings/77120
Forum: 454 Pyrosequencing 05-29-2015, 04:04 AM
Replies: 4
Views: 3,194
Posted By mps4208
I haven't heard anything yet, but we are hoping...

I haven't heard anything yet, but we are hoping the same thing.

Epicentre is slowly shifting everything to Illumina, at least Illumina picked up the Nextera.
Forum: 454 Pyrosequencing 05-18-2015, 09:18 AM
Replies: 1
Views: 2,121
Posted By mps4208
454 Junior XL+ sequencing reagents?

Does anyone have 454 Junior XL+ seq reagents/enzymes currently in your freezer? I need 1 kit but Roche hasn't been able to provide it for the past 2 months.
Forum: 454 Pyrosequencing 02-03-2015, 04:22 AM
Replies: 11
Views: 4,434
Posted By mps4208
11min 30sec

(It was expensive in time only as we got reagents replaced)
Roche tech support suggested since our rapid libraries were long 1,700bp (Agilent) that we might need 1-2 extra minutes during extension...
Forum: 454 Pyrosequencing 02-02-2015, 11:59 AM
Replies: 11
Views: 4,434
Posted By mps4208
Success on XL Junior!

I know this reply is way after the fact, hopefully you have had success by now. I write mostly because we were having the same issues and this seems to be the latest thread. We got the XL on the...
Forum: 454 Pyrosequencing 11-08-2013, 04:57 AM
Replies: 3
Views: 1,678
Posted By mps4208
Not specifically aqueous phase, but maybe the...

Not specifically aqueous phase, but maybe the following will be a start.

Matthias Meyer paper in 2008 has the emPCR primer sequences. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248761/
...
Forum: 454 Pyrosequencing 10-16-2013, 07:38 AM
Replies: 11
Views: 5,690
Posted By mps4208
Ahh, I like our little 454 GS Junior. 454 is...

Ahh, I like our little 454 GS Junior. 454 is still the best available for long reads.
Forum: 454 Pyrosequencing 08-27-2013, 05:52 AM
Replies: 3
Views: 1,348
Posted By mps4208
500ng used to be a small amount, but the other...

500ng used to be a small amount, but the other companies have managed to create more sensitive kits-Roche seems to be behind in that field.

500ng is because they assume that 1/2 is lost during...
Forum: 454 Pyrosequencing 08-27-2013, 04:25 AM
Replies: 3
Views: 1,348
Posted By mps4208
Many of my clients love the GS Junior, but it...

Many of my clients love the GS Junior, but it does have it's niche among sequencers. As long as you are aware of those at the beginning, you'll be happier with your outcome.

A few things to...
Forum: 454 Pyrosequencing 08-19-2013, 09:22 AM
Replies: 1
Views: 1,961
Posted By mps4208
Roche RL Standard reagent

Does anyone know what the Standard in the rapid library kit consists of? I'm wondering if it is just a set amount of Y-adaptors. Does any other company make anything similar? I know there are qPCR...
Forum: 454 Pyrosequencing 07-30-2013, 09:02 AM
Replies: 3
Views: 1,366
Posted By mps4208
We have the GS Junior and also use the a sticky...

We have the GS Junior and also use the a sticky seal tape, we haven't had any emulsions break so far. Our emulsions never really come in contact with the seal. I'm not sure how different it is for...
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