Forum: Bioinformatics
03-17-2017, 11:40 AM
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Replies: 7
Views: 5,658
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Forum: Bioinformatics
03-03-2017, 10:55 AM
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Replies: 7
Views: 5,658
Not back into the read, back into the header line
Hi, my goal is not to put them back into the sequences themselves but to put them at the end of the header line for each sequence in the fastq. Let me know if this is not clear and I can cut and...
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Forum: Bioinformatics
03-03-2017, 07:58 AM
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Replies: 7
Views: 5,658
Adding barcode indexes back into FASTQ headers?
Hi,
I have a set of fastq files from a fungal ITS2 amplicon run on a MiSeq. There are 3 corresponding fastq files: Read1, Read2 and an Index file. In the past, I have worked with fastq files...
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Forum: Illumina/Solexa
04-15-2016, 10:49 AM
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Replies: 1
Views: 2,824
Any input?
Hi, I'm curious if anyone has any input on the post I made a couple weeks ago. I would be grateful for some advice.
To clarify my main questions:
1) What is the best way to remove primers using...
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Forum: Illumina/Solexa
04-01-2016, 12:28 PM
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Replies: 1
Views: 2,824
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Forum: Illumina/Solexa
04-01-2016, 12:18 PM
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Replies: 4
Views: 4,103
Thanks
OK, I see. Thanks for the help.
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Forum: Illumina/Solexa
03-28-2016, 01:26 PM
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Replies: 4
Views: 4,103
GenoMax, thanks for the reply. The adapter...
GenoMax, thanks for the reply. The adapter trimming on the 3' end makes sense to me now. Thanks.
What I mean by the primer pads are best shown in the the supplement from Tremblay et al. 2015 (see...
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Forum: Illumina/Solexa
03-28-2016, 10:14 AM
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Replies: 4
Views: 4,103
Adapter trimming with BBduk
Hi, I am working with some MiSeq 16S and ITS2 amplicon sequence data generated by JGI. Previously I utilized the data after it was quality controlled and merged but now I am going back to the...
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