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Forum: Metagenomics 05-04-2011, 07:15 AM
Replies: 1
Views: 4,698
Posted By chrisaw01
Sample/library prep of DNA and RNA in a metagenomic sample

Hi all,

Many of the metagenomic studies I have seen published so far seem to focus on either RNA viruses OR bacterial communities (i.e., DNA). Can anyone point me to some protocols or published...
Forum: 454 Pyrosequencing 04-01-2011, 05:24 PM
Replies: 0
Views: 4,178
Posted By chrisaw01
cpb ratio

Does anyone have any experience in differences in optimal copy-per-bead ratios for 454 FLX when using cDNA libraries vs. standard DNA libraries?
Forum: General 11-05-2010, 11:25 AM
Replies: 1
Views: 1,753
Posted By chrisaw01
Whole genome sequencing primers

Hi,

I was wondering if anyone knew of a company that will generate whole genome sequencing primers for ABI/Sanger sequencing given I provide them with a whole genome sequence of a reference. I am...
Forum: Introductions 10-21-2010, 05:50 AM
Replies: 373
Views: 151,633
Posted By chrisaw01
Hi Zun, I also just started a RNA-seq...

Hi Zun,

I also just started a RNA-seq project and would be interested to hear how things progress for you.

Chris
Forum: Sample Prep / Library Generation 10-20-2010, 10:01 AM
Replies: 26
Views: 31,874
Posted By chrisaw01
Hi, Can anyone comment on what viral RNA would...

Hi,
Can anyone comment on what viral RNA would look like using the Bioanalyzer? Obviously you would not see rRNA peaks unless they were carry-over from the cells the virus was grown in?

Thanks
Forum: Introductions 10-20-2010, 07:04 AM
Replies: 373
Views: 151,633
Posted By chrisaw01
Hello, I am a Staff Scientist in a new...

Hello,

I am a Staff Scientist in a new genomics center for the U.S. Army. We using 454 and Illumina and our focus is on infectious disease genomics. I am pretty new to genomics and hoping to...
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