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Forum: Metagenomics

05-04-2011, 07:15 AM

Replies: 1

Sample/library prep of DNA and RNA in a metagenomic sample

Views: 4,698

Posted By chrisaw01

Sample/library prep of DNA and RNA in a metagenomic sample

Hi all,

Many of the metagenomic studies I have seen published so far seem to focus on either RNA viruses OR bacterial communities (i.e., DNA). Can anyone point me to some protocols or published...

Forum: 454 Pyrosequencing

04-01-2011, 05:24 PM

Replies: 0

cpb ratio

Views: 4,178

Posted By chrisaw01

cpb ratio

Does anyone have any experience in differences in optimal copy-per-bead ratios for 454 FLX when using cDNA libraries vs. standard DNA libraries?

Forum: General

11-05-2010, 11:25 AM

Replies: 1

Whole genome sequencing primers

Views: 1,753

Posted By chrisaw01

Whole genome sequencing primers

Hi,

I was wondering if anyone knew of a company that will generate whole genome sequencing primers for ABI/Sanger sequencing given I provide them with a whole genome sequence of a reference. I am...

Forum: Introductions

10-21-2010, 05:50 AM

Replies: 373

Sticky: Swing by and introduce yourself!

Views: 151,633

Posted By chrisaw01

Hi Zun, I also just started a RNA-seq...

Hi Zun,

I also just started a RNA-seq project and would be interested to hear how things progress for you.

Chris

Forum: Sample Prep / Library Generation

10-20-2010, 10:01 AM

Replies: 26

Total RNA quality (Bioanalyzer trace attached)

Views: 31,874

Posted By chrisaw01

Hi, Can anyone comment on what viral RNA would...

Hi,
Can anyone comment on what viral RNA would look like using the Bioanalyzer? Obviously you would not see rRNA peaks unless they were carry-over from the cells the virus was grown in?

Thanks

Forum: Introductions

10-20-2010, 07:04 AM

Replies: 373

Sticky: Swing by and introduce yourself!

Views: 151,633

Posted By chrisaw01

Hello, I am a Staff Scientist in a new...

Hello,

I am a Staff Scientist in a new genomics center for the U.S. Army. We using 454 and Illumina and our focus is on infectious disease genomics. I am pretty new to genomics and hoping to...

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