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Forum: Core Facilities 11-21-2012, 09:14 AM
Replies: 3
Views: 4,140
Posted By Exeplex
...though presumably this will depend on the...

...though presumably this will depend on the total target size for your library - XT2 format baits libraries are currently only available from Agilent for targets >500 kb total (which is rather odd...
Forum: Illumina/Solexa 11-24-2010, 08:15 AM
Replies: 0
Views: 1,573
Posted By Exeplex
Question Version 5 flowcell

Hi,

Does anyone have any details on the impending Illumina v5 flowcell (for the GAIIx), and how this differs from previous versions? I can't find anything in current posts about this, but...
Forum: Sample Prep / Library Generation 08-18-2010, 08:35 AM
Replies: 1
Views: 3,929
Posted By Exeplex
Hi, There is some useful information in the...

Hi,

There is some useful information in the MYselect manual (a system not dissimilar to Agilent's SureSelect), where they actually give you such details as the bait concentration and numbers of...
Forum: Sample Prep / Library Generation 08-18-2010, 07:39 AM
Replies: 3
Views: 4,323
Posted By Exeplex
The fixed length of the Agilent baits (120-mers)...

The fixed length of the Agilent baits (120-mers) means the design algorithm is rather simpler, but has the potential disadvantage that base composition will affect the hybridization kinetics of...
Forum: Sample Prep / Library Generation 10-29-2009, 07:12 AM
Replies: 1
Views: 6,505
Posted By Exeplex
Oligo concentrations

The concentration in the adapter mix is 15 uM - you can find this info in the Cronn et al 2008 paper in NAR, although you can also work it out from the Illumina protocol: for DNA fragmented to an...
Forum: Illumina/Solexa 10-29-2009, 06:52 AM
Replies: 24
Views: 27,897
Posted By Exeplex
Thanks Shivering, I'll check them out

Thanks Shivering, I'll check them out
Forum: Illumina/Solexa 10-26-2009, 10:31 AM
Replies: 24
Views: 27,897
Posted By Exeplex
Library-free addition of index sequences in circularized MIP captured sequences?

Hi ShiveringFire,

In my diagram the index is introduced by the reverse primer in the inverse PCR step (called the Capture_Slxa_Rev_Amp primer in the Turner paper), so you would need a unique...
Forum: Illumina/Solexa 10-05-2009, 03:35 PM
Replies: 24
Views: 27,897
Posted By Exeplex
Library-free addition of index sequences in circularized MIP captured sequences?

I haven't tried this, but in principle you should be able to carry out direct indexed sequencing from MIP-derived amplicons by incorporating a barcode into the middle of the reverse primer (between...
Forum: Sample Prep / Library Generation 08-17-2009, 03:34 AM
Replies: 7
Views: 11,498
Posted By Exeplex
Gel extraction

Scott's tip about not heating during gel dissolution is a good one - see the paper by Quail et al. (Nature Methods, December 2008) for more details - though the time needed for complete...
Forum: Illumina/Solexa 07-07-2009, 09:16 AM
Replies: 24
Views: 27,897
Posted By Exeplex
Multiplexing with custom adapters

Hi,

Re. the second part of this post, various groups (e.g. Cronn et al., NAR 2008; Harismendy & Frazer, Biotechniques 2009) have used custom adapters incorporating the barcode so that it becomes...
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