Forum: Core Facilities
11-21-2012, 09:14 AM
|
|
Replies: 3
Views: 4,140
...though presumably this will depend on the...
...though presumably this will depend on the total target size for your library - XT2 format baits libraries are currently only available from Agilent for targets >500 kb total (which is rather odd...
|
|
Forum: Illumina/Solexa
11-24-2010, 08:15 AM
|
|
Replies: 0
Views: 1,573
Version 5 flowcell
Hi,
Does anyone have any details on the impending Illumina v5 flowcell (for the GAIIx), and how this differs from previous versions? I can't find anything in current posts about this, but...
|
|
Forum: Sample Prep / Library Generation
08-18-2010, 08:35 AM
|
|
Replies: 1
Views: 3,929
Hi,
There is some useful information in the...
Hi,
There is some useful information in the MYselect manual (a system not dissimilar to Agilent's SureSelect), where they actually give you such details as the bait concentration and numbers of...
|
|
Forum: Sample Prep / Library Generation
08-18-2010, 07:39 AM
|
|
Replies: 3
Views: 4,323
|
|
Forum: Sample Prep / Library Generation
10-29-2009, 07:12 AM
|
|
Replies: 1
Views: 6,505
Oligo concentrations
The concentration in the adapter mix is 15 uM - you can find this info in the Cronn et al 2008 paper in NAR, although you can also work it out from the Illumina protocol: for DNA fragmented to an...
|
|
Forum: Illumina/Solexa
10-29-2009, 06:52 AM
|
|
Replies: 24
Views: 27,897
|
|
Forum: Illumina/Solexa
10-26-2009, 10:31 AM
|
|
Replies: 24
Views: 27,897
|
|
Forum: Illumina/Solexa
10-05-2009, 03:35 PM
|
|
Replies: 24
Views: 27,897
|
|
Forum: Sample Prep / Library Generation
08-17-2009, 03:34 AM
|
|
Replies: 7
Views: 11,498
Gel extraction
Scott's tip about not heating during gel dissolution is a good one - see the paper by Quail et al. (Nature Methods, December 2008) for more details - though the time needed for complete...
|
|
Forum: Illumina/Solexa
07-07-2009, 09:16 AM
|
|
Replies: 24
Views: 27,897
Multiplexing with custom adapters
Hi,
Re. the second part of this post, various groups (e.g. Cronn et al., NAR 2008; Harismendy & Frazer, Biotechniques 2009) have used custom adapters incorporating the barcode so that it becomes...
|
