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Forum: RNA Sequencing 02-04-2014, 07:49 AM
Replies: 0
Views: 1,106
Posted By seb330
RNAseq and qPCR don't agree, but only some genes

I've done RNAseq on bacterial samples for gene expression studies over a time course, and have been validating some of the results via qPCR, using the same RNA samples as we sent for sequencing. Most...
Forum: RNA Sequencing 02-12-2013, 11:02 AM
Replies: 2
Views: 1,320
Posted By seb330
qPCR to validate DGE

I'm designing some qPCR reactions to test some candidate genes before we get our RNA-seq results back. Does anyone have a good suggestion for a control gene to use for bacterial samples? I've heard...
Forum: RNA Sequencing 11-14-2012, 02:59 PM
Replies: 3
Views: 1,788
Posted By seb330
Thanks, rflrob! That definitely makes me feel...

Thanks, rflrob! That definitely makes me feel better about multiplexing these! Our genome is ~5Mb, so that would definitely be enough coverage based on your numbers. Are you able to see good, clear...
Forum: RNA Sequencing 11-13-2012, 11:35 AM
Replies: 3
Views: 1,788
Posted By seb330
Optimal coverage for RNA-seq analysis of DGE in bacteria

Hi all,

I am planning an experiment to look at DGE in a bacterial culture before and after nutrient depletion. We will be doing RNA-seq using an Illumina Hi-seq, and the facility has told us that...
Forum: Introductions 10-25-2012, 11:21 AM
Replies: 0
Hi!
Views: 633
Posted By seb330
Hi!

Hi all,

I'm a post-doc at Penn State University. A microbiologist/molecular biologist by training, I somehow got mixed up with a bunch of biophysicists that had already started a project to use...
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