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Forum: Academic/Non-Profit Jobs 08-27-2013, 05:07 PM
Replies: 0
Views: 1,682
Posted By dhinerfeld
Senior Manager of Genome Technologies at The Jackson Laboratory

Senior Manager, Genome Technologies
The Jackson Laboratory...
Forum: Sample Prep / Library Generation 08-31-2012, 08:51 AM
Replies: 1
Views: 1,574
Posted By dhinerfeld
Covaris E220 service contract

We have just purchased a Covaris system for fragmentation and am trying to determine if we need a service contract. My question is how ofter these systems require service? If anyone has experience...
Forum: Illumina/Solexa 06-20-2012, 01:26 PM
Replies: 0
Views: 2,210
Posted By dhinerfeld
Unhappy Illumina Cancer Panel problems

We are getting either no or low yield after the PCR when running this assay. One thing we have noticed is that during the long annealing of the initial primers, there is substantial evaporation even...
Forum: Sample Prep / Library Generation 06-24-2011, 09:22 AM
Replies: 7
Views: 12,166
Posted By dhinerfeld
We have seen this. If you go into the PCR on the...

We have seen this. If you go into the PCR on the high end of target amounts, in the late cycles of PCR the primers become limiting. At this point, when you anneal, you get many bubble molecules...
Forum: Illumina/Solexa 06-23-2011, 02:54 PM
Replies: 7
Views: 3,926
Posted By dhinerfeld
We haven't but will be soon. As I understand it,...

We haven't but will be soon. As I understand it, V3 will soon be the only option from Illumina. I actually have a question about how much library to load on the V3 kits to get in the range of 800k...
Forum: Academic/Non-Profit Jobs 03-25-2011, 09:37 AM
Replies: 0
Views: 1,676
Posted By dhinerfeld
Manager, High-throughput sequencing and gene expression lab

There is an opportunity at The Jackson Laboratory in Bar Harbor, Maine for a Manager of the High-throughput Sequencing and Gene Expression Service. The service operates a series of technologies...
Forum: Illumina/Solexa 11-05-2010, 10:08 AM
Replies: 9
Views: 5,114
Posted By dhinerfeld
Does anyone actually have the Caliper system up...

Does anyone actually have the Caliper system up and running in their lab for mRNAseq or DNAseq library construction?
Forum: Sample Prep / Library Generation 11-05-2010, 10:07 AM
Replies: 46
Views: 23,823
Posted By dhinerfeld
Has anyone used the Pippin for mRNAseq libraries...

Has anyone used the Pippin for mRNAseq libraries using the Illumina-type protocol. On the surface, it should work just fine, but we have had some struggles with getting the PCR to work well after...
Forum: Illumina/Solexa 08-16-2010, 08:44 AM
Replies: 5
Views: 2,979
Posted By dhinerfeld
The site prep guide for the HiSeq says that the...

The site prep guide for the HiSeq says that the lab temp must be 19-25C and that the temp cannot vary more than +/-2C during a run.
Forum: Illumina/Solexa 08-13-2010, 09:54 AM
Replies: 5
Views: 2,979
Posted By dhinerfeld
Lab temperature and HiSeq performance

We are just preparing our lab for the installation of a HiSeq. We are in an older building so have some concern about the +/- 2C temperature requirements during the length of the run. I am...
Forum: Illumina/Solexa 06-10-2010, 01:58 PM
Replies: 17
Views: 9,817
Posted By dhinerfeld
If you do run into issues with a run, Illumina is...

If you do run into issues with a run, Illumina is much more likely to replace your reagents if you have run the phiX control. Just a warning.
Forum: Academic/Non-Profit Jobs 01-29-2010, 02:17 PM
Replies: 1
Views: 1,591
Posted By dhinerfeld
Bioinformatics position at The Jackson Laboratory

Manager, Computational Sciences, Statistics and Analysis

A unique opportunity exists as Manager of the Computational Sciences, Statistics and Analysis (CS-SA) Group. This key position manages all...
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