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Forum: Sample Prep / Library Generation 02-05-2013, 04:11 AM
Replies: 10
Views: 3,852
Posted By SLeigh-Brown
Looking at your bioanalyzer trace, Rosmano, I...

Looking at your bioanalyzer trace, Rosmano, I believe that these are unlikey to be bubble products. We had a lot of trouble with bubble products a few months ago using the Truseq RNA library prep...
Forum: Illumina/Solexa 01-07-2013, 04:19 AM
Replies: 0
Views: 2,188
Posted By SLeigh-Brown
Question Low cluster density of Truseq "high throughput" libraries - both DNA and RNA

Hi genomics folks,

Has anyone got any experience in clustering Illumina Truseq RNA 'HT' (high throughput) and Truseq DNA HT libraries?

I work in an NGS service, and we recently tried out the...
Forum: Sample Prep / Library Generation 09-27-2012, 01:57 AM
Replies: 12
Views: 16,184
Posted By SLeigh-Brown
Truseq on 100ng input

Hi everyone,

Illumina currently only sell a Nextera kit for DNA library preps.

The Nextera enzyme will only work on double-stranded DNA, so if you are considering testing it for RNAseq, I...
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