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  • pmiguel
    Senior Member
    • Aug 2008
    • 2328
    #16
    Originally posted by GenoMax View Post
    Do you see this on every flowcell? All the way down to the images like Phillip posted above?



    The bubbles were not there in the images from the two flowcells I looked at. I will check with the lab folks here to see if they have anything to add.
    Could you see what was causing the high error rates in the tiles you looked at?

    --
    Phillip

    Comment

    • GW_OK
      Senior Member
      • Sep 2009
      • 411
      #17
      Originally posted by GenoMax View Post
      Do you see this on every flowcell? All the way down to the images like Phillip posted above?

      The bubbles were not there in the images from the two flowcells I looked at. I will check with the lab folks here to see if they have anything to add.
      Not always. Very intermittent, but it does exist. And I can find those tiles and see the dark bubbles circles as Phillip showed. I'd go find some right now but we dump thumbnails after the run and I don't have anything running right now. I will say I haven't seen it in any of the rapid runs we've done so far.

      Comment

      • pmiguel
        Senior Member
        • Aug 2008
        • 2328
        #18
        My instrument is a HiSeq2500 (upgraded from a HiSeq2000). What instruments do you guys have?

        Comment

        • GW_OK
          Senior Member
          • Sep 2009
          • 411
          #19
          Same here.

          Comment

          • GenoMax
            Senior Member
            • Feb 2008
            • 7142
            #20
            Both HiSeq 2000 and 2500.
            Last edited by GenoMax; 04-09-2013, 09:59 AM.

            Comment

            • pmiguel
              Senior Member
              • Aug 2008
              • 2328
              #21
              Illumina tech support just mentioned that there is some bubble-related issue with "swath and tile drop out" at some HiSeq 2500 sites. They have launched an internal investigation.

              --
              Phillip

              Comment

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