I just tried to determine miRNA features, I computed most but I still don't know how to compute the structure features like the stem length, base paring probability, largest bulge, gap ratio,,etc I tried to get my answer with the Vienna package but I couldn't. What do you suggest. Actually, I tried to extract all the possible features of the secondary structure of miRNA, I used perl and Bioperl but I didn't find any function or package to help me to get the stem length, base paring probability, largest bulge, gap ratio..etc
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
