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  • hanshart
    Member
    • Nov 2011
    • 27

    ENSEMBL biotype tRNA and non-coding RNA

    Hello, I have two questions concerning the ENSEMBL annotation files (GTFs for human, mouse).

    1. Can anyone explain to me why there is no gene_biotype "tRNA" (but "rRNA","Mt_rRNA", "Mt_tRNA") annotated in the annotation files for human and mouse (ENSEMBL version 70), although it is documented? See http://www.ensembl.org/info/docs/genebuild/ncrna.html
    Ok, GENCODE (should use the same database as ENSEMBL) does not list it -> http://www.gencodegenes.org/gencode_biotypes.html
    Is tRNA described by other biotypes?

    2. Does anyone know how "complete" the ENSEMBL non-coding RNA annotations are in contrast to specialized non-coding RNA databases (especially for lincRNA)? If I apply an analysis to output percentage of mapped reads per biotype based only on the ENSEMBL annotation file, whould this analysis be representative for the non-coding RNAs (for human and for mouse)?

    Thank you
    Last edited by hanshart; 05-17-2013, 10:06 AM. Reason: null
  • sudders
    Member
    • Dec 2011
    • 32

    #2
    Hi,

    Did you ever get this sorted? I can't seem to find any annotation of tRNAs in human ensembl.

    Ian
    ---

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