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  • JdeBruin
    Member
    • Jun 2013
    • 25

    #16
    This is indeed a RHEL 6.x system

    I have not done as suggested with "libbam.a" is this included in the Casava dir?

    Thanks

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #17
      Originally posted by JdeBruin View Post
      This is indeed a RHEL 6.x system

      I have not done as suggested with "libbam.a" is this included in the Casava dir?

      Thanks
      Looking at your original log, is the error being produced during "samtools" build? We built casava-1.8.2 a long time ago so I do not remember if "samtools" was included in the installer or we already had it installed.

      The "libbam.a" is part of the samtools and should get built as a part of the samtools package.

      Comment

      • JdeBruin
        Member
        • Jun 2013
        • 25

        #18
        Yes I get the error when samtools are being build as part of a Casava installation.
        I found libbam.a in opt/samtools-0.1.8/ but I believe due to the fact that the error is produced while samtools are being built, libbam.a should be placed when the build (make command) has been completed succefully...

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #19
          Is samtools being built successfully? Do you see the samtools executable?

          Comment

          • JdeBruin
            Member
            • Jun 2013
            • 25

            #20
            Samtools fails to build, the configure pass no problem but the make command fails.

            Comment

            • GenoMax
              Senior Member
              • Feb 2008
              • 7142

              #21
              Have you installed both "zlib" and "zlib-devel"?

              Comment

              • JdeBruin
                Member
                • Jun 2013
                • 25

                #22
                Hi,

                Yes I have installed both:
                Version : 1.2.3 Vendor: Red Hat, Inc.

                Comment

                • GenoMax
                  Senior Member
                  • Feb 2008
                  • 7142

                  #23
                  Would you mind getting the samtools package from here (as an aside) and checking if it compiles? http://sourceforge.net/projects/samtools/files/

                  Comment

                  • JdeBruin
                    Member
                    • Jun 2013
                    • 25

                    #24
                    Hi will do so

                    Comment

                    • JdeBruin
                      Member
                      • Jun 2013
                      • 25

                      #25
                      I have done so and I am still unable to "make" succesfully.

                      Error:

                      /root/apps/tmp_samtools/samtools-0.1.19/phase.c:475: undefined reference to `gzopen64'
                      bedcov.o: In function `main_bedcov':
                      /root/apps/tmp_samtools/samtools-0.1.19/bedcov.c:69: undefined reference to `gzopen64'
                      libbam.a(bam_import.o): In function `__bam_get_lines':
                      /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:76: undefined reference to `gzopen64'
                      libbam.a(bam_import.o): In function `sam_open':
                      /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:472: undefined reference to `gzopen64'
                      libbam.a(bam_import.o): In function `sam_header_read2':
                      /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:126: undefined reference to `gzopen64'
                      libbam.a(bedidx.o):/root/apps/tmp_samtools/samtools-0.1.19/bedidx.c:107: more undefined references to `gzopen64' follow

                      What is this gzopen64 ?

                      Comment

                      • GenoMax
                        Senior Member
                        • Feb 2008
                        • 7142

                        #26
                        Originally posted by JdeBruin View Post
                        I have done so and I am still unable to "make" succesfully.

                        Error:

                        /root/apps/tmp_samtools/samtools-0.1.19/phase.c:475: undefined reference to `gzopen64'
                        bedcov.o: In function `main_bedcov':
                        /root/apps/tmp_samtools/samtools-0.1.19/bedcov.c:69: undefined reference to `gzopen64'
                        libbam.a(bam_import.o): In function `__bam_get_lines':
                        /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:76: undefined reference to `gzopen64'
                        libbam.a(bam_import.o): In function `sam_open':
                        /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:472: undefined reference to `gzopen64'
                        libbam.a(bam_import.o): In function `sam_header_read2':
                        /root/apps/tmp_samtools/samtools-0.1.19/bam_import.c:126: undefined reference to `gzopen64'
                        libbam.a(bedidx.o):/root/apps/tmp_samtools/samtools-0.1.19/bedidx.c:107: more undefined references to `gzopen64' follow

                        What is this gzopen64 ?
                        That means that your zlib is probably not installed correctly. Are you using the RH supplied zlib?

                        Where exactly is your zlib.h installed?
                        Last edited by GenoMax; 06-04-2013, 03:58 AM.

                        Comment

                        • JdeBruin
                          Member
                          • Jun 2013
                          • 25

                          #27
                          Hey,

                          I have used the RH installed zlib I have also downloaded the latest zlib from zlib.org and compiled from source.

                          Probably have to uninstall all zlib and start over?

                          Comment

                          • GenoMax
                            Senior Member
                            • Feb 2008
                            • 7142

                            #28
                            Originally posted by JdeBruin View Post
                            Hey,

                            I have used the RH installed zlib I have also downloaded the latest zlib from zlib.org and compiled from source.

                            Probably have to uninstall all zlib and start over?
                            I would say remove the zlib you downloaded from zlib.org and try the compile again.

                            Comment

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