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  • lewewoo
    Member
    • Apr 2011
    • 60

    closest TSS of given peaks

    Hi I have a list of ChipSeq peaks, what are the fastest way to find the closest TSS in the given length (for example, 1kb, 2kb... 10kb); Any tools or Perl scripts for this? Thanks a lot!
  • Dario1984
    Senior Member
    • Jun 2011
    • 166

    #2
    There is a Bioconductor package called ChIPpeakAnno. If you know the R language, you should use it.

    Comment

    • lewewoo
      Member
      • Apr 2011
      • 60

      #3
      Thanks!
      I will try it for sure!

      Comment

      • crazyhottommy
        Senior Member
        • Apr 2012
        • 187

        #4
        or you can use http://cistrome.org/ap/
        based on Galaxy.

        under the cistrome tool box
        go to Integrative Analysis, click peak2gene: Peak Center Annotation

        you can get a list of genes close to your peaks.

        Comment

        • gene_x
          Senior Member
          • May 2010
          • 108

          #5
          bedtools?

          see: http://bedtools.readthedocs.org/en/l...s/closest.html

          Comment

          • kadircaner
            Member
            • Jun 2011
            • 13

            #6
            you can also use GREAT - great.stanford.edu

            Comment

            • ashuchawla
              Member
              • Jan 2012
              • 38

              #7
              I have to do it the other-way round - I have to find the peaks near all the genes in Human. I have the peak list with start and end positions. I also have a gene list with start transcription and end transcription .

              Any pointers on how to do that would be really appreciated

              Comment

              • crazyhottommy
                Senior Member
                • Apr 2012
                • 187

                #8
                as suggested, have a try for bedtools http://bedtools.readthedocs.org/en/l...s/closest.html


                Originally posted by ashuchawla View Post
                I have to do it the other-way round - I have to find the peaks near all the genes in Human. I have the peak list with start and end positions. I also have a gene list with start transcription and end transcription .

                Any pointers on how to do that would be really appreciated

                Comment

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