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  • shelleyDNASEQ
    Junior Member
    • Jul 2013
    • 2
    #1

    Big Dye Left out all night

    I am just a relief DNA Sequencer and filling in for this week. I'm new to the game...

    I left some big dye out last night-about 15 hours at 25C-is it still good?

    Suggestions?

    Thanks!
    Tags: big dye, spoiled terminator
    • shelleyDNASEQ
      Junior Member
      • Jul 2013
      • 2
      #2
      my own answer

      I tested the stuff out on my pGEM control and it looked fine, so I decided to use it.

        Comment

        • The Spith
          Junior Member
          • Jan 2013
          • 2
          #3
          Divide it into small aliquots so you don't have to constantly freeze-thaw it. That tends to be thing that degrades enzyme the most. It also means that if you leave it out overnight you will at worst only ruin a small amount, and not your whole stock.

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