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  • qingchang
    Junior Member
    • Jul 2010
    • 3
    #1

    WGA techniques

    I am new in NGS. I am wondering if anybody knows the difference between 2 WGA methods:

    MALBAC of Sunney Xie

    vs

    Rubicon's PicoPlex

    I thought they are similar, but I could be wrong.
    Tags: None
    • Genohub
      Registered Vendor
      • Mar 2013
      • 210
      #2
      WGA, MALBAC and Rubicon

      Our blog post describes several single cell sequencing approaches. For more details check out their patents:

      Rubicon

      MALBAC

        Comment

        • raimotanzi
          Junior Member
          • May 2009
          • 4
          #3
          They are all good when you have "few" cells, like 10 or more, but all suffer when you have a "single" cell. Mostly you will experience high allelic dropout and high noise in Copy number profiles. Currently, the method which is reported by multiple comparative articles to be the best performer for "single" cell WGA is Ampli1 http://www.siliconbiosystems.com/ampli1-wga-kit
          PLOS ONE | https://doi.org/10.1371/journal.pone.0193689 March 1, 2018
          PLOS ONE | DOI:10.1371/journal.pone.0171566 February 16, 2017

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